Kuroyanagi Misato, Yamamoto Akiko, Shimizu Nahoko, Toi Ayako, Inomata Tomonori, Takeda Akira, Kuroyanagi Yoshimitsu
a Plastic and Aethetic Surgery, School of Medicine , Kitasato University , 1-15-1, Kitasato, Minami-ku, Sagamihara , Kanagawa 252-0373 , Japan.
J Biomater Sci Polym Ed. 2014;25(12):1253-65. doi: 10.1080/09205063.2014.926579. Epub 2014 Jun 18.
Anti-adhesive products need to be designed while considering the concept of wound healing. Two main events must proceed simultaneously: facilitating wound healing in surgically excised tissue, as well as preventing injured tissue from adhering to the surrounding tissue. The present study aimed to develop an anti-adhesive spongy sheet composed of hyaluronic acid and collagen (Col) containing epidermal growth factor, and to investigate the potential of this spongy sheet using an in vitro wound surface model (placing a spongy sheet on a fibroblast-incorporating Col gel sheet) and an in vitro inter-tissue model (placing a spongy sheet between two fibroblast-incorporating Col gel sheets). These in vitro experiments demonstrated that this spongy sheet effectively stimulates fibroblasts to release an increased amount of vascular endothelial growth factor and hepatocyte growth factor, which are essential for wound healing to proceed succesfully. In addition, anti-adhesive performance of this spongy sheet was evaluated in animal experiments using Sprague Dawley rats. Under anesthesia, a 1 cm × 2 cm segment of peritoneum was superficially excised from walls, and the cecum was then abraded by scraping with a scalpel blade over a 1 cm × 2 cm area. A piece of spongy sheet was placed on the peritoneal defect. Both defects were placed in contact, and the incision was closed by suturing. Peritoneal condition was evaluated after one week. This spongy sheet was capable of facilitating the wound healing of surgically excised tissue and preventing surgically excised tissue from adhering to surrounding tissues.
抗粘连产品的设计需要考虑伤口愈合的概念。两个主要事件必须同时进行：促进手术切除组织的伤口愈合，以及防止受伤组织与周围组织粘连。本研究旨在开发一种由含有表皮生长因子的透明质酸和胶原蛋白（Col）组成的抗粘连海绵片，并使用体外伤口表面模型（将海绵片放置在含有成纤维细胞的Col凝胶片上）和体外组织间模型（将海绵片放置在两个含有成纤维细胞的Col凝胶片之间）来研究这种海绵片的潜力。这些体外实验表明，这种海绵片能有效刺激成纤维细胞释放更多的血管内皮生长因子和肝细胞生长因子，这些因子对于伤口顺利愈合至关重要。此外，使用Sprague Dawley大鼠进行动物实验评估了这种海绵片的抗粘连性能。在麻醉下，从腹壁表面切除1 cm×2 cm的腹膜段，然后用手术刀在1 cm×2 cm的区域刮擦使盲肠擦伤。将一片海绵片放置在腹膜缺损处。使两个缺损处接触，然后通过缝合关闭切口。一周后评估腹膜情况。这种海绵片能够促进手术切除组织的伤口愈合，并防止手术切除组织与周围组织粘连。
