Carvalho Paulo D, Hackbart Katherine S, Bender Robb W, Baez Giovanni M, Dresch Ana R, Guenther Jerry N, Souza Alex H, Fricke Paul M
Department of Dairy Science, University of Wisconsin, Madison, Wisconsin, USA.
Department of Dairy Science, University of Wisconsin, Madison, Wisconsin, USA.
Theriogenology. 2014 Aug;82(3):481-9. doi: 10.1016/j.theriogenology.2014.05.011. Epub 2014 May 21.
Our objective was to compare several experimental preparations of a single injection of long-acting recombinant bovine FSH (rbFSH; types A and B) to a porcine pituitary-derived FSH (Folltropin) to superovulate Holstein dairy heifers. Nonlactating, nonpregnant virgin Holstein heifers (n = 56) aged 12 to 15 months were randomly assigned to one of four superstimulatory treatments. Beginning at a random stage of the estrous cycle, all follicles greater than 5 mm were aspirated. Thirty-six hours later, heifers received an intravaginal P4 device and superstimulatory treatments were initiated. Treatments were (1) 300 mg of pituitary-derived FSH (Folltropin) administered in eight decreasing doses over a period of 3.5 days; (2) a single injection of 50 μg of A-rbFSH; (3) a single injection of 100 μg of A-rbFSH; and (4) a single injection of 50 μg of B-rbFSH. All heifers received 25 mg PGF2α at 48 and 72 hours after the insertion of P4 device. At 84 hours after insertion, P4 devices were removed, and ovulation was induced 24 hours later with hCG (2500 IU). Heifers were inseminated at 12 and 24 hours after hCG treatment. The number of ovulatory follicles was greatest for heifers treated with Folltropin and B50-rbFSH, least for heifers treated with A50-rbFSH, and was intermediate for heifers treated with A100-rbFSH (25.7 ± 3.2, 18.9 ± 3.2, 5.9 ± 0.9, and 16.6 ± 3.1, respectively; P < 0.001). The number of corpora lutea was greatest for heifers treated with Folltropin, B50-rbFSH, and A100-rbFSH, and least for heifers treated with A50-rbFSH (19.1 ± 2.4, 16.1 ± 3.0, 15.9 ± 2.9, and 2.6 ± 0.9, respectively; P < 0.001). The number of good-quality embryos differed among treatments and was greatest for heifers treated with B50-rbFSH, Folltropin, and A100-rbFSH and least for heifers treated with A50-rbFSH (7.6 ± 2.4, 6.5 ± 1.7, 4.3 ± 1.5, and 0.8 ± 0.5, respectively; P < 0.001). In conclusion, a single injection of a preparation of long-acting rbFSH (either 100 μg of A-rbFSH or 50 μg of B-rbFSH but not 50 μg of A-rbFSH) produced similar superovulatory responses resulting in the production of good-quality embryos when compared with a pituitary-derived FSH preparation administered twice daily for 4 days. More studies using different types of cattle and different doses of rbFSH are needed to confirm the findings reported in this preliminary study.
我们的目标是比较单次注射长效重组牛促卵泡素（rbFSH；A 型和 B 型）的几种实验制剂与猪垂体来源的促卵泡素（Folltropin）对荷斯坦奶牛小母牛进行超数排卵的效果。12 至 15 月龄、非泌乳、未怀孕的处女荷斯坦小母牛（n = 56）被随机分配到四种超刺激处理之一。在发情周期的随机阶段开始，抽吸所有直径大于 5 毫米的卵泡。36 小时后，给小母牛放置阴道内 P4 装置并开始超刺激处理。处理方式如下：（1）在 3.5 天内分八次递减剂量注射 300 毫克垂体来源的促卵泡素（Folltropin）；（2）单次注射 50 微克 A 型 rbFSH；（3）单次注射 100 微克 A 型 rbFSH；（4）单次注射 50 微克 B 型 rbFSH。所有小母牛在放置 P4 装置后 48 小时和 72 小时接受 25 毫克 PGF2α。在放置后 84 小时，取出 P4 装置，并在 24 小时后用 hCG（2500 国际单位）诱导排卵。小母牛在 hCG 处理后 12 小时和 24 小时进行授精。用 Folltropin 和 B50 - rbFSH 处理的小母牛排卵卵泡数量最多，用 A50 - rbFSH 处理的小母牛最少，用 A100 - rbFSH 处理的小母牛居中（分别为 25.7 ± 3.2、18.9 ± 3.2、5.9 ± 0.9 和 16.6 ± 3.1；P < 0.001）。用 Folltropin、B50 - rbFSH 和 A100 - rbFSH 处理的小母牛黄体数量最多，用 A50 - rbFSH 处理的小母牛最少（分别为 19.1 ± 2.4、16.1 ± 3.0、15.9 ± 2.9 和 2.6 ± 0.9；P < 0.001）。优质胚胎数量在各处理间存在差异，用 B50 - rbFSH、Folltropin 和 A100 - rbFSH 处理的小母牛最多，用 A50 - rbFSH 处理的小母牛最少（分别为 7.6 ± 2.4、6.5 ± 1.7、4.3 ± 1.5 和 0.8 ± 0.5；P < 0.001）。总之，与每天两次、连续 4 天注射垂体来源的促卵泡素制剂相比，单次注射长效 rbFSH 制剂（100 微克 A 型 rbFSH 或 50 微克 B 型 rbFSH，但不是 50 微克 A 型 rbFSH）产生了相似的超数排卵反应，从而产生了优质胚胎。需要更多使用不同类型牛和不同剂量 rbFSH 的研究来证实本初步研究报告的结果。
