Ma Xiao-Long, Ye Ju-Xiang, Su Jing, Qi Fei-Fei, Meng Qing-Yang, Shi Xue-Ying
Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Department of Pathology, Peking University Third Hospital, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Pathol Res Pract. 2014 Sep;210(9):543-7. doi: 10.1016/j.prp.2014.05.004. Epub 2014 May 22.
Lymph node (LN) retrieval is important for proper staging of colorectal carcinoma. Although various assistant techniques were recommended to facilitate LN identification, most of them were unavoidably time-consuming, resource intensive and costly. We prepared a modified GEWF solution (RE-GEWF) by use of recycled alcohol and a familiar dye, eosin and investigated its efficacy on 55 colorectal carcinoma specimens. Of the 55 studied cases, 33 of them with <12 LNs (Group A) and 22 with ≥12 LNs were detected (Group B) before RE-GEWF treatment. All were subsequently treated with RE-GEWF for 14-16h and were inspected again for LNs. The number of LNs revealed before and after RE-GEWF treatment was 539 and 476 respectively. The mean number of LNs per cases increased from 9.80±6.27 to 18.43±8.77. Twelve accessory LN metastases were found in 9 cases. Upgrade of pN stage was only present in 7 of the Group A cases. The results show that RE-GEWF is as effective as other reported LN revealing solutions. Use of RE-GEWF not only can assure the quality of LN detection, but also minimize the cost and reduce the release of waste.
淋巴结（LN）清扫对于结直肠癌的准确分期至关重要。尽管推荐了各种辅助技术来促进LN的识别，但其中大多数不可避免地耗时、资源密集且成本高昂。我们使用回收酒精和一种常见染料伊红制备了改良的GEWF溶液（RE-GEWF），并在55例结直肠癌标本上研究了其效果。在RE-GEWF处理前，55例研究病例中，33例有<12个LN（A组），22例有≥12个LN（B组）。随后所有病例均用RE-GEWF处理14 - 16小时，并再次检查LN。RE-GEWF处理前后发现的LN数量分别为539个和476个。每例LN的平均数量从9.80±6.27增加到18.43±8.77。在9例中发现12处副LN转移。pN分期的升级仅在A组的7例病例中出现。结果表明，RE-GEWF与其他报道的LN显示溶液效果相同。使用RE-GEWF不仅可以确保LN检测的质量，还能将成本降至最低并减少废物排放。
