Svec A, Horák L, Novotný J, Lysy P
Department of Pathology, Faculty Hospital Královské Vinohrady and Third Medical Faculty, Charles University, Prague, Czech Republic.
Eur J Surg Oncol. 2006 May;32(4):426-9. doi: 10.1016/j.ejso.2006.02.002. Epub 2006 Mar 13.
To verify an impact of a lymph node revealing solution (LRS) on the number of lymph nodes (LN) revealed in colorectal resection specimens.
Fifty-nine cases of rectosigmoid carcinomas divided into two subgroups-stage pT2&3 (20 cases) and ypT2&3 (with pre-operative radiotherapy-39 cases)-were formalin fixed for 42-72 h, serially sectioned and re-fixed in LRS containing ethanol, diethyl ether, glacial acetic acid, and formalin for 24 h. The number of LNs was matched to a control group consisting of 34 cases of colorectal carcinoma, 14 pT2&3 and 20 ypT2&3 rectosigmoid carcinomas examined routinely. Results were statistically tested by Student's t-test.
In the study group the total number of revealed LNs was 869, range 1-48, average 14.7 per specimen, median 15. In the control group the number of LNs was 200, range 0-13, average 5.9 per specimen, median 6. The difference between the study and the control group was statistically significant (P = 0.05).
Using LRS leads to a significant increase in the number of revealed LNs in colorectal resection specimens and makes it possible to find more than 12 LNs in a majority of stage pT2 and pT3 adenocarcinomas.
验证淋巴结显示液（LRS）对结直肠切除标本中显示的淋巴结数量的影响。
59例直肠乙状结肠癌分为两个亚组——pT2&3期（20例）和ypT2&3期（术前接受放疗，39例），用福尔马林固定42 - 72小时，连续切片，然后在含有乙醇、乙醚、冰醋酸和福尔马林的LRS中重新固定24小时。将淋巴结数量与一个对照组进行匹配，该对照组由34例结直肠癌组成，其中14例pT2&3期和20例ypT2&3期直肠乙状结肠癌进行常规检查。结果采用学生t检验进行统计学分析。
研究组显示的淋巴结总数为869个，范围为1 - 48个，平均每个标本14.7个，中位数为15个。对照组的淋巴结数量为200个，范围为0 - 13个，平均每个标本5.9个，中位数为6个。研究组与对照组之间的差异具有统计学意义（P = 0.05）。
使用LRS可使结直肠切除标本中显示的淋巴结数量显著增加，并有可能在大多数pT2和pT3期腺癌中发现超过12个淋巴结。
