Li Yanhong, Yao Li, Liu Fei, Hong Jia, Chen Lin, Zhang Beilei, Zhang Wei
Department of Gynecology and Obstetrics, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
The Helmholtz Sino‑German Research Laboratory for Cancer, Department of Pathology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China.
Int J Mol Med. 2014 Aug;34(2):491-8. doi: 10.3892/ijmm.2014.1813. Epub 2014 Jun 17.
Serous ovarian cancer is a major gynecologic malignancy with a poor 5‑year survival rate. However, little is known regarding the behavior and genetics of ovarian tumorigenesis. MicroRNAs (miRNAs) have been shown to be dysregulated in ovarian carcinomas. To assess the miRNA expression profiles in serous ovarian cancer, we defined the patterns of miRNA expression in 100 formalin‑fixed, paraffin‑embedded ovarian cancer tissues blocks as well as 50 corresponding normal oviduct tissues using miRNA microarray. MiRNA expression profiling showed that 63 miRNAs were downregulated and 43 miRNAs were upregulated in serous ovarian cancer tissues compared with control tissues. The expression of five dysregulated miRNAs was validated using quantitative polymerase chain reaction (RT‑qPCR). GO term and pathway analysis revealed that the biological process of the cell cycle was significantly enriched and the MAPK signaling pathway was highly involved in the progression of ovarian cancer. The results suggested that the aberrant expression of miRNAs is involved in ovarian carcinogenesis and thus these miRNAs may function as diagnostic and prognostic biomarkers.
浆液性卵巢癌是一种主要的妇科恶性肿瘤，5年生存率较低。然而，关于卵巢肿瘤发生的行为和遗传学知之甚少。微小RNA（miRNA）已被证明在卵巢癌中表达失调。为了评估浆液性卵巢癌中的miRNA表达谱，我们使用miRNA微阵列定义了100个福尔马林固定、石蜡包埋的卵巢癌组织块以及50个相应正常输卵管组织中的miRNA表达模式。miRNA表达谱分析显示，与对照组织相比，浆液性卵巢癌组织中有63个miRNA表达下调，43个miRNA表达上调。使用定量聚合酶链反应（RT-qPCR）验证了5个失调miRNA的表达。基因本体（GO）术语和通路分析显示，细胞周期的生物学过程显著富集，丝裂原活化蛋白激酶（MAPK）信号通路高度参与卵巢癌的进展。结果表明，miRNA的异常表达参与了卵巢癌的发生，因此这些miRNA可能作为诊断和预后生物标志物发挥作用。
