Adegbolagun O M, Emikpe B O, Woranola I O, Ogunremi Y
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Ibadan.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
Afr Health Sci. 2013 Dec;13(4):970-6. doi: 10.4314/ahs.v13i4.16.
Resistance to most antimalarial drugs has encouraged the use of herbal preparations along with prescribed orthodox drugs.
To investigate effect of co-administration of aqueous extract of T. occidentalis leaves; commonly used as antimalarial and haematinic agent in Nigeria and artesunate using P. berghei animal model.
In vivo curative antiplasmodial effect of T. occidentalis (200mg/kg) alone and combination with artesunate (2mg/kg) were evaluated using albino mice infected with 10(6) parasitized erythrocytes of P. berghei intraperitoneally. The haematological parameters: haemoglobin level, red blood cells and white blood cells and packed cell volume were monitored using standard methods.
Aqueous extract of T. occidentalis, artesunate and the combination gave 72.17±4.07%, 70.43± 4.27% and 85.43±3.65% reduction in parasitaemia after 48hours respectively. A significant enhancement of the PCV was obtained with the coadministration of artesunate and aqueous extract (p< 0.01). Similar trends were also observed with heamatological parameters at 72hours of administration.
This study revealed a synergistic effect of the co-administration on parasite clearance rate of P. berghei infection in mice, with a significant enhancement of haematological parameters within 48 hours of administration. This indicates a rapid rate of recovery from plasmodial infections with the co-administration.
对大多数抗疟药物的耐药性促使人们将草药制剂与规定的正统药物一起使用。
使用伯氏疟原虫动物模型，研究尼日利亚常用作抗疟和补血剂的西方罗勒叶水提取物与青蒿琥酯联合使用的效果。
使用腹腔注射感染10⁶个伯氏疟原虫寄生红细胞的白化小鼠，评估西方罗勒（200mg/kg）单独使用以及与青蒿琥酯（2mg/kg）联合使用的体内抗疟疗效。使用标准方法监测血液学参数：血红蛋白水平、红细胞、白细胞和血细胞比容。
48小时后，西方罗勒叶水提取物、青蒿琥酯及其组合分别使疟原虫血症降低72.17±4.07%、70.43±4.27%和85.43±3.65%。青蒿琥酯与水提取物联合使用可显著提高血细胞比容（p<0.01）。给药72小时后，血液学参数也观察到类似趋势。
本研究揭示了联合给药对小鼠伯氏疟原虫感染的寄生虫清除率具有协同作用，给药后48小时内血液学参数显著提高。这表明联合给药可使疟原虫感染快速恢复。
