DU Yue, Hou Ling, Guo Jinjie, Sun Tingting, Wang Xiuli, Wu Yubin
Department of Pediatric Nephrology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Exp Ther Med. 2014 May;7(5):1130-1134. doi: 10.3892/etm.2014.1595. Epub 2014 Feb 28.
The aim of this study was to investigate the expression of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) in the serum, urine and renal tissues of children with acute kidney injury (AKI) and Henoch-Schönlein purpura nephritis (A-on-C). A prospective single-center evaluation of the serum, urine and renal NGAL and KIM-1 levels was performed in a cohort of children. Blood and 5-ml urine samples were collected from each patient for the analysis of NGAL and KIM-1 levels using an ELISA. In addition, the expression of NGAL and KIM-1 in the kidney was examined using immunohistochemistry in patients with A-on-C and HSPN. The expression of serum cystatin C, β2-macroglobulin and serum creatinine (SCr), as well as urinary β2-MG and SCr, in the patients with A-on-C was significantly higher than that of HSPN patients, and the expression of NGAL and KIM-1 in the serum and urine in the A-on-C patients was also significantly higher than that of HSPN patients. However, there were no significant differences in the urine protein levels between the two groups. NGAL and KIM-1 were expressed in renal tubular epithelial cells, and the expression of NGAL and KIM-1 in the A-on-C patients was significantly higher than that in HSPN patients. In addition, the urine NGAL and KIM-1 levels were negatively correlated with glomerular filtration rate, but there was no significant correlation between the urine NGAL/KIM-1 and urine protein levels. The changes in serum and urine NGAL and KIM-1 levels may be applied to the diagnosis of A-on-C.
本研究旨在探讨中性粒细胞明胶酶相关脂质运载蛋白（NGAL）和肾损伤分子-1（KIM-1）在急性肾损伤（AKI）和紫癜性肾炎（A-on-C）患儿血清、尿液及肾组织中的表达情况。对一组儿童进行了血清、尿液及肾脏NGAL和KIM-1水平的前瞻性单中心评估。从每位患者采集血液和5毫升尿液样本，采用酶联免疫吸附测定法（ELISA）分析NGAL和KIM-1水平。此外，采用免疫组织化学法检测A-on-C和紫癜性肾炎（HSPN）患者肾脏中NGAL和KIM-1的表达。A-on-C患者血清胱抑素C、β2-微球蛋白和血清肌酐（SCr）以及尿β2-MG和SCr的表达显著高于HSPN患者，A-on-C患者血清和尿液中NGAL和KIM-1的表达也显著高于HSPN患者。然而，两组间尿蛋白水平无显著差异。NGAL和KIM-1在肾小管上皮细胞中表达，A-on-C患者中NGAL和KIM-1的表达显著高于HSPN患者。此外，尿NGAL和KIM-1水平与肾小球滤过率呈负相关，但尿NGAL/KIM-1与尿蛋白水平之间无显著相关性。血清和尿液中NGAL和KIM-1水平的变化可用于A-on-C的诊断。
