Zhu Lei, Chen Yuxuan, Liu Chongdong, Deng Haiteng, Zhang Nawei, Wang Shengdian, Zhang Zhenyu
Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing 100020, P.R. China.
The Rockefeller University, New York, NY 10065, USA.
Exp Ther Med. 2014 May;7(5):1332-1336. doi: 10.3892/etm.2014.1558. Epub 2014 Feb 18.
Preeclampsia (PE) is considered to be a potentially fatal complication during pregnancy. However, no effective laboratory assessment has been developed to enable early diagnosis and monitoring of PE. The present study aimed to identify differentially expressed transthyretin (TTR) during severe PE and evaluate TTR as a possible biomarker of this disease. TTR levels were determined in the different gestational weeks of normal pregnancy (before 20 weeks, n=41; after 20 weeks, n=39) using enzyme-linked immunosorbent assay (ELISA). TTR concentrations in pregnant females with severe PE (n=43) were compared with those in healthy matched control subjects (n=37) using western blot analysis and ELISA. The median TTR concentration during severe PE in each month of gestation was significantly lower than the concentrations recorded during normal pregnancy. TTR levels in females with severe PE were significantly downregulated compared with the control subjects (P<0.001; area under the curve, 0.834-0.967). Thus, TTR may be used as a potential biomarker of PE.
子痫前期（PE）被认为是妊娠期一种潜在的致命并发症。然而，尚未开发出有效的实验室评估方法来实现对子痫前期的早期诊断和监测。本研究旨在鉴定重度子痫前期期间差异表达的转甲状腺素蛋白（TTR），并评估TTR作为该疾病可能的生物标志物。使用酶联免疫吸附测定（ELISA）法测定正常妊娠不同孕周（20周前，n = 41；20周后，n = 39）的TTR水平。采用蛋白质免疫印迹分析和ELISA法，将重度子痫前期孕妇（n = 43）的TTR浓度与健康匹配对照受试者（n = 37）的TTR浓度进行比较。重度子痫前期各孕周期间的TTR浓度中位数显著低于正常妊娠期间记录的浓度。与对照受试者相比，重度子痫前期女性的TTR水平显著下调（P<0.001；曲线下面积，0.834 - 0.967）。因此，TTR可能用作子痫前期的潜在生物标志物。
