花生四烯酸的脂氧合酶代谢产物对孕酮和前列腺素E2生成的刺激作用。

Stimulation of progesterone and prostaglandin E2 production by lipoxygenase metabolites of arachidonic acid.

作者信息

Wang J, Yuen B H, Leung P C

机构信息

Department of Obstetrics/Gynaecology, University of British Columbia, Vancouver, Canada.

出版信息

FEBS Lett. 1989 Feb 13;244(1):154-8. doi: 10.1016/0014-5793(89)81182-2.

DOI:10.1016/0014-5793(89)81182-2

PMID:2494061

Abstract

The role of several lipoxygenase metabolites of arachidonic acid in the action of luteinizing hormone-releasing hormone (LHRH) on ovarian hormone production was investigated. Like LHRH, treatment of rat granulosa cells with 5-HETE, 5-HPETE, 12-HETE, 15-HETE or 15-HPETE stimulated progesterone (P) and prostaglandin E2 (PGE2) production. 12-HEPE was most potent and stimulated P and PGE2 equally well. By contrast, 5-HETE stimulated P better than PGE2, while 15-HETE was a potent stimulator of PGE2 but not of P. Stimulation of P and PGE2 by LHRH or 12-O-tetradecanoylphorbol 13-acetate (TPA) was further augmented by several HETEs and HPETEs. Like protein kinase C, arachidonic acid metabolites appear to mediate the multiple actions of LHRH in the ovary.

摘要

研究了花生四烯酸的几种脂氧合酶代谢产物在促黄体生成激素释放激素（LHRH）对卵巢激素产生作用中的角色。与LHRH一样，用5-羟基二十碳四烯酸（5-HETE）、5-氢过氧化二十碳四烯酸（5-HPETE）、12-羟基二十碳四烯酸（12-HETE）、15-羟基二十碳四烯酸（15-HETE）或15-氢过氧化二十碳四烯酸（15-HPETE）处理大鼠颗粒细胞可刺激孕酮（P）和前列腺素E2（PGE2）的产生。12-氢过氧化二十碳四烯酸（12-HEPE）作用最强，对P和PGE2的刺激效果相同。相比之下，5-HETE对P的刺激优于PGE2，而15-HETE是PGE2的有效刺激剂，但对P无刺激作用。几种羟基二十碳四烯酸（HETEs）和氢过氧化二十碳四烯酸（HPETEs）进一步增强了LHRH或12-O-十四酰佛波醇-13-乙酸酯（TPA）对P和PGE2的刺激作用。与蛋白激酶C一样，花生四烯酸代谢产物似乎介导了LHRH在卵巢中的多种作用。