Meghji S, Sandy J R, Scutt A M, Harvey W, Harris M
Joint Department of Oral and Maxillo-Facial Surgery, University College, London, U.K.
Prostaglandins. 1988 Aug;36(2):139-49. doi: 10.1016/0090-6980(88)90301-2.
We have studied the effect of leukotrienes, (LT): B4, C4, D4 and E4 and the hydroxyeicosatetraenoic acids (HETEs) 5-HETE and 12-HETE on bone resorption in vitro. Resorption was measured by colorimetric assay of calcium released from neonatal mouse calvaria maintained in organ culture for 72h. All the LTs and HETEs stimulated bone resorption, with optimum responses at picomolar or nanomolar concentrations. The responses were biphasic, with a decreasing effect at higher concentrations. In contrast, prostaglandin E2 (PGE2) stimulated resorption only at 10nM and above. Indomethacin partially inhibited resorption by LTB4, LTC4 and LTD4, but did not affect resorption stimulated by LTE4, 5-HETE and 12-HETE. These results indicate that lipoxygenase products of arachidonic acid are highly potent bone resorbing factors and may play an important role in the localised bone loss associated with inflammatory lesions.
我们研究了白三烯（LT）：B4、C4、D4和E4以及羟基二十碳四烯酸（HETE）5-HETE和12-HETE对体外骨吸收的影响。通过比色法测定在器官培养中维持72小时的新生小鼠颅骨释放的钙来测量骨吸收。所有的白三烯和羟基二十碳四烯酸均刺激骨吸收，在皮摩尔或纳摩尔浓度时反应最佳。反应呈双相性，在较高浓度时作用减弱。相比之下，前列腺素E2（PGE2）仅在10nM及以上浓度时刺激骨吸收。吲哚美辛部分抑制LTB4、LTC4和LTD4引起的骨吸收，但不影响LTE4、5-HETE和12-HETE刺激的骨吸收。这些结果表明，花生四烯酸的脂氧合酶产物是高效的骨吸收因子，可能在与炎症性病变相关的局部骨质流失中起重要作用。
