Olate J, Jorquera H, Purcell P, Codina J, Birnbaumer L, Allende J E
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
FEBS Lett. 1989 Feb 13;244(1):188-92. doi: 10.1016/0014-5793(89)81190-1.
Xenopus laevis oocytes are cells ideally suited to the study of signal transduction and of the G-proteins that are involved in this process. A X. laevis cDNA library in lambda gt10 has been screened with a mixture of three oligonucleotide probes designed to detect sequences found in various mammalian alpha-subunits of G-proteins. One of these clones has been purified through tertiary screening and the DNA insert has been sequenced. This clone was found to include the total sequence coding for a 354 amino acid protein that is 89% identical to the sequence of alpha-subunit of rat Go. The differences with the mammalian protein were clustered in amino acids 290-315, which have been postulated to define the region interacting with the receptor and effector molecule. The homology with the alpha-subunits of other mammalian G-proteins is lower (65-70% to Gi and 42% to Gs). On this basis, this clone can be classified as Go-like.
