Carruthers Christopher A, Dearth Christopher L, Reing Janet E, Kramer Caroline R, Gagne Darcy H, Crapo Peter M, Garcia Onelio, Badhwar Amit, Scott Jeffrey R, Badylak Stephen F
1 Department of Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania.
Tissue Eng Part A. 2015 Jan;21(1-2):35-44. doi: 10.1089/ten.TEA.2014.0095. Epub 2014 Sep 29.
Acellular dermal matrices (ADMs) have been commonly used in expander-based breast reconstruction to provide inferolateral prosthesis coverage. Although the clinical performance of these biologic scaffold materials varies depending on a number of factors, an in-depth systematic characterization of the host response is yet to be performed. The present study evaluates the biochemical composition and structure of two ADMs, AlloDerm(®) Regenerative Tissue Matrix and AlloMax™ Surgical Graft, and provides a comprehensive spatiotemporal characterization in a porcine model of tissue expander breast reconstruction.
Each ADM was characterized with regard to thickness, permeability, donor nucleic acid content, (residual double-stranded DNA [dsDNA]), and growth factors (basic fibroblast growth factor [bFGF], vascular endothelial growth factor [VEGF], and transforming growth factor-beta 1 [TGF-β1]). Cytocompatibility was evaluated by in vitro cell culture on the ADMs. The host response was evaluated at 4 and 12 weeks at various locations within the ADMs using established metrics of the inflammatory and tissue remodeling response: cell infiltration, multinucleate giant cell formation, extent of ADM remodeling, and neovascularization.
AlloMax incorporated more readily with surrounding host tissue as measured by earlier and greater cell infiltration, fewer foreign body giant cells, and faster remodeling of ADM. These findings correlated with the in vitro composition and cytocompatibility analysis, which showed AlloMax to more readily support in vitro cell growth.
AlloMax and AlloDerm demonstrated distinct remodeling characteristics in a porcine model of tissue expander breast reconstruction.
脱细胞真皮基质（ADM）已普遍用于基于扩张器的乳房重建，以提供假体的下外侧覆盖。尽管这些生物支架材料的临床性能因多种因素而异，但尚未对宿主反应进行深入的系统表征。本研究评估了两种ADM（AlloDerm®再生组织基质和AlloMax™手术移植物）的生化组成和结构，并在组织扩张器乳房重建的猪模型中提供了全面的时空表征。
对每种ADM的厚度、渗透性、供体核酸含量（残留双链DNA[dsDNA]）和生长因子（碱性成纤维细胞生长因子[bFGF]、血管内皮生长因子[VEGF]和转化生长因子-β1[TGF-β1]）进行表征。通过在ADM上进行体外细胞培养来评估细胞相容性。使用炎症和组织重塑反应的既定指标，在4周和12周时评估ADM内不同位置的宿主反应：细胞浸润、多核巨细胞形成、ADM重塑程度和新血管形成。
通过更早和更大程度的细胞浸润、更少的异物巨细胞以及更快的ADM重塑来衡量，AlloMax与周围宿主组织的融合更迅速。这些发现与体外组成和细胞相容性分析相关，后者表明AlloMax更易于支持体外细胞生长。
在组织扩张器乳房重建的猪模型中，AlloMax和AlloDerm表现出不同的重塑特征。
