Kooy A, Weber R F, Ooms M P, Vreeburg J T
Department of Endocrinology, Growth and Reproduction, Erasmus University, Rotterdam, The Netherlands.
J Endocrinol. 1989 Feb;120(2):261-8. doi: 10.1677/joe.0.1200261.
The effects of the transplantable purely prolactin-secreting tumour 7315b on serum gonadotrophins were studied in adult rats. Possible contributions of the adrenals to the tumour-induced inhibition of serum LH and FSH were evaluated. The suppressive actions of tumour 7315b on serum gonadotrophins in gonadectomized plus adrenalectomized male and female rats were compared. Within 4 weeks after inoculation of tumour 7315b in intact male rats very high levels of prolactin and decreased serum levels of gonadotrophins and testosterone were recorded. At autopsy reduced weights of testes and accessory sex organs and slightly increased adrenal weights were found. In addition, in animals treated with a small testosterone-filled capsule after castration, tumour 7315b reduced serum concentrations of LH and FSH. Adrenalectomy did not prevent this suppressive action of the tumour on the post-castration rise of serum gonadotrophins. Suppression of serum gonadotrophins during hyperprolactinaemia was greater in gonadectomized plus adrenalectomized female rats than in male rats, indicating that the degree of the tumour-induced suppression of LH and FSH after castration is determined to a large extent by the sex of the animal. The purely prolactin-secreting tumour 7315b has therefore been shown to be a suitable model for studying the effects of severe hyperprolactinaemia on the pituitary-gonadal axis in rats.
在成年大鼠中研究了可移植的纯催乳素分泌肿瘤7315b对血清促性腺激素的影响。评估了肾上腺对肿瘤诱导的血清促黄体生成素（LH）和促卵泡生成素（FSH）抑制作用的可能贡献。比较了肿瘤7315b对去性腺加肾上腺切除的雄性和雌性大鼠血清促性腺激素的抑制作用。在完整雄性大鼠接种肿瘤7315b后4周内，记录到催乳素水平非常高，血清促性腺激素和睾酮水平降低。尸检时发现睾丸和附属生殖器官重量减轻，肾上腺重量略有增加。此外，在阉割后用填充小剂量睾酮的胶囊治疗的动物中，肿瘤7315b降低了血清LH和FSH浓度。肾上腺切除术并不能阻止肿瘤对阉割后血清促性腺激素升高的这种抑制作用。高催乳素血症期间，去性腺加肾上腺切除的雌性大鼠血清促性腺激素的抑制作用比雄性大鼠更大，这表明阉割后肿瘤诱导的LH和FSH抑制程度在很大程度上取决于动物的性别。因此，纯催乳素分泌肿瘤7315b已被证明是研究严重高催乳素血症对大鼠垂体-性腺轴影响的合适模型。
