对带有SnAvi标签的蛋白质进行靶向纯化。

Targeted purification of SnAvi-tagged proteins.

作者信息

Schäffer Ursula, Baumeister Ralf, Schulze Ekkehard

机构信息

Institute for Biology 3, University of Freiburg, Schänzlestr. 4, 79104, Freiburg, Germany,

出版信息

Methods Mol Biol. 2014;1177:163-74. doi: 10.1007/978-1-4939-1034-2_13.

DOI:10.1007/978-1-4939-1034-2_13

PMID:24943322

Abstract

Tandem affinity purification (TAP) is a powerful technique to identify protein complex members. The modular composition of TAP-tags allows two sequential protein enrichment steps and thereby drastically reduces the amount of contaminants. Here, we describe the application of the SnAvi-tag-a TAP-tag useful in different expression systems. Due to its modular composition, this tag is multifunctional and facilitates among others the in vivo visualization of tagged proteins and their cell type specific activation.

摘要

串联亲和纯化（TAP）是一种用于鉴定蛋白质复合物成员的强大技术。TAP标签的模块化组成允许进行两个连续的蛋白质富集步骤，从而大大减少污染物的量。在这里，我们描述了SnAvi标签（一种在不同表达系统中有用的TAP标签）的应用。由于其模块化组成，该标签具有多种功能，尤其有助于对标记蛋白进行体内可视化及其细胞类型特异性激活。