以纤维素结合模块3为亲和标签对重组蛋白进行纯化。

Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag.

作者信息

Wang Dongmei, Hong Jiong

机构信息

School of Life Science, University of Science and Technology of China, Huangshan Road 443, 230026, Hefei, Anhui, People's Republic of China.

出版信息

Methods Mol Biol. 2014;1177:35-45. doi: 10.1007/978-1-4939-1034-2_3.

DOI:10.1007/978-1-4939-1034-2_3

PMID:24943312

Abstract

Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.

摘要

易于操作且低成本的蛋白质纯化方法对于在生物经济中发挥重要作用的常用酶的大规模生产具有很高的需求。利用CBM3（3型纤维素结合模块）作为亲和标签，开发了一种低成本且快速的重组蛋白纯化系统。本方案描述了以CBM3作为亲和标签，纯化在毕赤酵母中表达的CBM3融合蛋白和无标签蛋白的方法。