Ortega Lorena, Garcia-Anguita Alicia, Riestra Pía, Ortega Henar, Soriano-Guillén Leandro, Lasunción Miguel A, de Oya Manuel, Garcés Carmen
Lipid Metabolism Research Laboratory, IIS-Fundación Jiménez Díaz, Madrid, Spain.
Servicio de Bioquímica-Investigación, Hospital Ramón y Cajal Madrid, and CIBER de Fisiología de la Obesidad y Nutrición (CIBERobn), Spain.
Steroids. 2014 Oct;88:15-8. doi: 10.1016/j.steroids.2014.06.007. Epub 2014 Jun 16.
Puberty is associated with decreased insulin sensitivity. Sexual hormones have been related with the onset of insulin resistance, but their relationship with non-esterified fatty acids (NEFA) remains unexplored. The aim of this study was to evaluate circulating NEFA levels in population-based samples of prepubertal children and adolescents and to analyze the association of NEFA with obesity, insulin resistance, and sexual hormones in adolescents.
The studied population included 854 randomly selected 6-8-year-old children and 822 children aged 12-16years. NEFA levels were determined using a commercial kit. Testosterone and estradiol levels were determined by RIA, and insulin and sex hormone binding protein by IRMA. HOMA was calculated as an indicator of insulin resistance.
NEFA levels were lower in adolescents than in 6-8-year-old children, and decreased progressively with age between 12-year-olds and 16-year-olds. No significant differences in NEFA levels were observed between obese and non-obese adolescents. NEFA were not correlated with insulin or HOMA in 12-16-year-old girls, and appear negatively correlated with these variables in boys. Insulin and HOMA were negatively correlated with SHBG levels in both sexes adjusting by age but NEFA levels were not.
NEFA levels decrease with age in adolescents and are not significantly increased in obese children, supporting the fact that the decreased insulin sensitivity at this age is not affecting NEFA metabolism. Although SHBG is related to insulin and HOMA independently of age in both sexes, SHBG levels are not associated with NEFA.
青春期与胰岛素敏感性降低有关。性激素与胰岛素抵抗的发生有关，但其与非酯化脂肪酸（NEFA）的关系仍未得到探索。本研究的目的是评估青春期前儿童和青少年群体样本中的循环NEFA水平，并分析青少年中NEFA与肥胖、胰岛素抵抗和性激素的关联。
研究人群包括854名随机选取的6至8岁儿童和822名12至16岁儿童。使用商业试剂盒测定NEFA水平。通过放射免疫分析法测定睾酮和雌二醇水平，通过免疫放射分析法测定胰岛素和性激素结合蛋白。计算HOMA作为胰岛素抵抗的指标。
青少年的NEFA水平低于6至8岁儿童，且在12岁至16岁之间随年龄逐渐降低。肥胖和非肥胖青少年的NEFA水平未观察到显著差异。在12至16岁女孩中，NEFA与胰岛素或HOMA无相关性，而在男孩中似乎与这些变量呈负相关。在按年龄调整后，胰岛素和HOMA在两性中均与SHBG水平呈负相关，但NEFA水平与SHBG水平无关。
青少年的NEFA水平随年龄降低，肥胖儿童中NEFA水平未显著升高，这支持了该年龄段胰岛素敏感性降低不影响NEFA代谢这一事实。尽管SHBG在两性中均独立于年龄与胰岛素和HOMA相关，但SHBG水平与NEFA无关。
