Pjechová M, Hernychova L, Tomašec P, Wilkinson G W, Vojtěšek B
Klin Onkol. 2014;27 Suppl 1:S116-20. doi: 10.14735/amko20141s116.
Protein phosphorylation is a key regulator in cellular signaling pathways. It is involved in most cellular events in which interplay between phosphatases and kinases strictly controls bio-logical processes, such as differentiation, proliferation and apoptosis. Altered or defective signaling pathways often result in various diseases, emphasizing the importance of studying the phosphoproteome. The abundance of phosphoproteins in the proteome is often very low, which requires specific and highly sensitive approaches. By using quantitative proteomics methods, we are able to analyze changes in abundance of proteins and their posttranslational modifications and then changes in signaling pathways. In this review, we describe quantitative proteomics methods, which could be used for study of phosphoproteins and their connection in signaling pathways.
蛋白质磷酸化是细胞信号通路中的关键调节因子。它参与了大多数细胞活动，其中磷酸酶和激酶之间的相互作用严格控制着生物过程，如分化、增殖和凋亡。信号通路的改变或缺陷常常导致各种疾病，这凸显了研究磷酸化蛋白质组的重要性。蛋白质组中磷酸化蛋白质的丰度通常非常低，这需要特定且高度灵敏的方法。通过使用定量蛋白质组学方法，我们能够分析蛋白质丰度及其翻译后修饰的变化，进而分析信号通路的变化。在本综述中，我们描述了可用于研究磷酸化蛋白质及其在信号通路中联系的定量蛋白质组学方法。
