Zahradníková M, Hernychova L, Vojtěšek B, Novotný M V
Klin Onkol. 2014;27 Suppl 1:S121-8. doi: 10.14735/amko20141s121.
Glycomics and glycoproteomics represent relatively new directions in detail analyses of complex bio-logical media. These areas of increasing importance to cancer research complement the more established genomic profiling and proteomics. Glycoproteins are being increasingly recognized as important in cellular interactions and adhesion. Structural alterations of their glycan moieties seem to occur in different cancer conditions. We review current directions in glycomic profiling and glycoproteomic investigations of bio-logical fluids and tissues pertaining to cancer. The used methods rely on capillary separation techniques, mass spectrometry, and the glycan and lectin arrays. They all show considerable promise for new diagnostic and prognostic measurements.
糖组学和糖蛋白质组学是复杂生物介质详细分析中相对较新的方向。这些对癌症研究日益重要的领域补充了更为成熟的基因组分析和蛋白质组学。糖蛋白在细胞相互作用和黏附中的重要性日益得到认可。其聚糖部分的结构改变似乎发生在不同的癌症状态中。我们综述了与癌症相关的生物体液和组织的糖组分析及糖蛋白质组研究的当前方向。所使用的方法依赖于毛细管分离技术、质谱以及聚糖和凝集素阵列。它们在新的诊断和预后测量方面都显示出相当大的前景。
