Krivánová M, Zelenák I
Vet Med (Praha). 1989 Feb;34(2):89-95.
The fermentation equipment RUSITEC (Rumen Simulation Technique) is a simplified model of the rumen which stimulates the conditions inside the rumen, enables to keep the normal microbial population of the rumen for a long time under strictly controllable conditions, and enables a permanent monitoring of fermentation processes. Trials were conducted to see whether this model of "artificial rumen" can be used to determine the nutritive value of untreated beech sawdust and beech sawdust treated hydrobarothermically at the pressure of 1.4 MPa and impregnated with 1% H2SO4. The digestibility of the dry matter and fibre components was higher in the treated beech sawdust than in the untreated sawdust. In both reaction vessels, the amounts of acetic, propionic and butyric acids as well as the total VFA, were higher during the fermentation of treated beech sawdust, as compared with the diet with raw beech sawdust. The increased output of VFA, especially the acetic and butyric acids, resulted in a proportional increase in the output of gases, especially carbon dioxide and methane, as compared with the production of gases in rumens digesting untreated beech sawdust. In comparison with other in-vitro methods, this model of fermentation equipment characterizes the nutritive values of various kinds of feed in a more thorough way: using this equipment, the simulation technique has proceeded much closer to reality.
发酵设备RUSITEC（瘤胃模拟技术）是瘤胃的简化模型，它能模拟瘤胃内的条件，在严格可控的条件下使瘤胃微生物种群长时间保持正常，并能对发酵过程进行持续监测。开展试验以探究这种“人工瘤胃”模型是否可用于测定未处理的山毛榉锯末以及在1.4 MPa压力下经湿热处理并用1%硫酸浸渍的山毛榉锯末的营养价值。处理后的山毛榉锯末中干物质和纤维成分的消化率高于未处理的锯末。在两个反应容器中，与含生山毛榉锯末的日粮相比，处理后的山毛榉锯末发酵过程中乙酸、丙酸和丁酸的量以及挥发性脂肪酸总量均更高。与消化未处理山毛榉锯末的瘤胃中气体产生量相比，挥发性脂肪酸产量增加，尤其是乙酸和丁酸，导致气体产量成比例增加，尤其是二氧化碳和甲烷。与其他体外方法相比，这种发酵设备模型能更全面地表征各类饲料的营养价值：使用该设备，模拟技术已更接近实际情况。
