Fonseca Belchiolina Beatriz, Beletti Marcelo Emílio, de Melo Roberta Torres, Mendonça Eliane Pereira, Coelho Letícia Ríspoli, Nalevaiko Priscila Christen, Rossi Daise Aparecida
Laboratório de Biotecnologia Animal Aplicada Faculdade de Medicina Veterinária Universidade Federal de Uberlândia UberlândiaMG Brazil ; Instituto de Ciências Biomédicas Universidade Federal de Uberlândia UberlândiaMG Brazil.
Instituto de Ciências Biomédicas Universidade Federal de Uberlândia UberlândiaMG Brazil.
Braz J Microbiol. 2014 May 19;45(1):76-9. doi: 10.1590/s1517-83822014000100011. eCollection 2014.
This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
本研究评估了空肠弯曲菌穿透商用鸡蛋壳孔隙并在鸡蛋内部定殖的能力，这可能成为人类感染的一个风险因素。此外，本研究还评估了该细菌在商用鸡蛋中的存活和活力情况。将鸡蛋与感染空肠弯曲菌的木屑接触，以检查细菌的通过情况。同时，将细菌直接接种到气室中，以评估其在蛋黄中的活力。为了确定蛋清和鸡蛋的受精情况是否会干扰细菌的进入和存活，我们使用了不同浓度的蛋清以及无特定病原体（SPF）鸡蛋和商用鸡蛋。与受污染木屑接触三小时后，在SPF（受精）鸡蛋中检测到了空肠弯曲菌，但在未受精的商用鸡蛋中未检测到。通过多重聚合酶链反应（PCR）对在SPF鸡蛋中分离出的菌落进行鉴定，并通过随机扩增多态性DNA-PCR（RAPD-PCR）验证菌株之间的相似性。该细菌能在商用鸡蛋和SPF鸡蛋的不同浓度蛋清中生长。直接接种到气室中的商用鸡蛋中未检测到空肠弯曲菌，但在木屑中测试的所有时间段内该细菌均具有活力。本研究表明，食用感染空肠弯曲菌的商用鸡蛋对人类健康不构成潜在风险。
