Kaczorek Ewa, Bielicka-Daszkiewicz Katarzyna, Héberger Károly, Kemény Sándor, Olszanowski Andrzej, Voelkel Adam
Institute of Chemical Technology and Engineering Poznan University of Technology Poznan Poland.
Chemical Research Center Hungarian Academy of Sciences Budapest Hungary.
Braz J Microbiol. 2014 May 19;45(1):117-26. doi: 10.1590/S1517-83822014005000029. eCollection 2014.
Diesel oil biodegradation by different bacteria-yeast-rhamnolipids consortia was tested. Chromatographic analysis of post-biodegradation residue was completed with chemometric tools (ANOVA, and a novel ranking procedure based on the sum of ranking differences). These tools were used in the selection of the most effective systems. The best results of aliphatic fractions of diesel oil biodegradation were observed for a yeast consortia with Aeromonas hydrophila KR4. For these systems the positive effect of rhamnolipids on hydrocarbon biodegradation was observed. However, rhamnolipids addition did not always have a positive influence on the biodegradation process (e.g. in case of yeast consortia with Stenotrophomonas maltophila KR7). Moreover, particular differences in the degradation pattern were observed for lower and higher alkanes than in the case with C22. Normally, the best conditions for "lower" alkanes are Aeromonas hydrophila KR4 + emulsifier independently from yeasts and e.g. Pseudomonas stutzeri KR7 for C24 alkane.
测试了不同细菌 - 酵母 - 鼠李糖脂联合体对柴油的生物降解作用。利用化学计量工具（方差分析以及基于排名差异总和的新型排名程序）完成了生物降解后残留物的色谱分析。这些工具用于选择最有效的系统。对于嗜水气单胞菌KR4与酵母的联合体，观察到柴油生物降解脂肪族馏分的最佳结果。对于这些系统，观察到鼠李糖脂对烃类生物降解的积极作用。然而，添加鼠李糖脂并不总是对生物降解过程有积极影响（例如在嗜麦芽窄食单胞菌KR7与酵母的联合体中）。此外，与C22情况相比，观察到低碳和高碳烷烃在降解模式上存在特定差异。通常，“低碳”烷烃的最佳条件是嗜水气单胞菌KR4 + 乳化剂，与酵母无关，而对于C24烷烃，例如斯氏假单胞菌KR7是最佳条件。
