Plommet M, Plommet A M
Station de Pathologie de la Reproduction, INRA, Centre de Tours-Nouzilly, France.
Ann Rech Vet. 1989;20(1):73-81.
Living vaccines induce immunity by lymphoid organs colonization that persists for a while before subsiding. Brucella abortus 19 vaccine injected into the footpad of mice was shown to colonize the popliteal lymph node and the spleen for at least 15 days before progressive disappearance. Administration of an immune serum before footpad vaccination restricted the colonization to the popliteal lymph node. The serum did not interfere with immunity when this immunity was measured by the virulent challenge spleen count method at least 90 days after vaccination. Immunity induced by lymph node restricted vaccination was very efficient 90 or 180 days after vaccination and was not boosted by a second injection administered 90 days after the first. This immunity was however about 20% lower, expressed in log10 spleen counts, than immunity induced by a non-restricted systemic vaccination which in addition was boosted by a recall. Restricted vaccination may mainly trigger the T-cell limb of immunity, whereas systemic vaccination triggers both humoral and cellular effectors.
活疫苗通过在淋巴器官定殖来诱导免疫，这种定殖会持续一段时间后消退。将流产布鲁氏菌19疫苗注射到小鼠足垫后，发现其在腘淋巴结和脾脏中定殖至少15天，之后逐渐消失。在足垫接种疫苗前给予免疫血清，可将定殖限制在腘淋巴结。当在接种疫苗至少90天后通过强毒攻击脾脏计数法测量免疫时，该血清不干扰免疫。由淋巴结限制接种诱导的免疫在接种后90天或180天非常有效，且在首次接种90天后进行的第二次注射不会增强这种免疫。然而，以log10脾脏计数表示，这种免疫比非限制全身接种诱导的免疫低约20%，此外，非限制全身接种还可通过再次接种增强免疫。限制接种可能主要触发免疫的T细胞分支，而全身接种则触发体液和细胞效应器。
