Research Center in Gastroenterology and Hepatology, Craiova 200638, Romania.
Research Center in Gastroenterology and Hepatology, Craiova 200638, Romania ; Laboratory of Human Genomics University of Medicine and Pharmacy of Craiova, Craiova 200638, Romania.
Endosc Ultrasound. 2013 Apr;2(2):86-91. doi: 10.4103/2303-9027.117692.
Angiogenesis is a crucial event for pancreatic carcinogenesis, and it also plays an important role in chronic pancreatitis. The aim of our study was to evaluate the mRNA expression of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in chronic inflammatory or malignant pancreatic pathology in order to elucidate the differences in expression patterns and potential clinical implications.
Thirty-five patients who had undergone endoscopic ultrasonography followed by endoscipic ultrasound-guided fine needle aspiration (EUS-FNA) of focal pancreatic masses were included in the study. VEGF and EGFR mRNA expression levels in the samples collected by EUS-FNA were analyzed using quantitative real-time polymerase chain reaction (PCR).
VEGF expression was detected in all chronic pancreatitis and adenocarcinoma samples and in only 62.5% of pancreatic neuroendocrine tumors. EGFR expression was detected in only 40% of the chronic pancreatitis cases, 76.9% of adenocarcinomas and in 50% of pancreatic neuroendocrine tumors. Both VEGF and EGFR mRNA levels were significantly higher in pancreatic ductal adenocarcinoma than those in normal tissue. VEGF expression inversely correlated with pancreatic ductal adenocarcinoma size, while EGFR expression was related to local invasiveness of adenocarcinoma.
Both VEGF and EGFR mRNA expression in EUS-FNA samples may be used as a diagnostic marker associated with invasiveness in patients with pancreatic adenocarcinoma.
血管生成是胰腺癌发生的关键事件，在慢性胰腺炎中也起着重要作用。我们的研究目的是评估血管内皮生长因子（VEGF）和表皮生长因子受体（EGFR）mRNA 在慢性炎症或恶性胰腺病理中的表达，以阐明表达模式的差异及其潜在的临床意义。
本研究纳入了 35 名因局灶性胰腺肿块而行内镜超声检查（EUS）后行 EUS 引导下细针抽吸（EUS-FNA）的患者。通过定量实时聚合酶链反应（PCR）分析 EUS-FNA 采集样本中的 VEGF 和 EGFR mRNA 表达水平。
VEGF 在所有慢性胰腺炎和腺癌样本中均有表达，而仅在 62.5%的胰腺神经内分泌肿瘤中检测到 EGFR 表达。EGFR 在 40%的慢性胰腺炎病例、76.9%的腺癌和 50%的胰腺神经内分泌肿瘤中仅检测到 EGFR 表达。VEGF 和 EGFR mRNA 水平在胰腺导管腺癌中均显著高于正常组织。VEGF 表达与胰腺导管腺癌的大小呈负相关，而 EGFR 表达与腺癌的局部侵袭性相关。
EUS-FNA 样本中的 VEGF 和 EGFR mRNA 表达均可作为与胰腺腺癌侵袭性相关的诊断标志物。
