Thompson J S, Saxena S K, Sharp J G
Omaha Veterans Administration Medical Center, NE.
Arch Surg. 1989 Apr;124(4):454-7. doi: 10.1001/archsurg.1989.01410040064014.
Patching intestinal defects with adjacent serosal surfaces results in the growth of new intestinal mucosa. Since polyamine biosynthesis is associated with cellular growth and differentiation, it may be important in this regenerative process. Our aim was to determine the effect of eflorithine (difluoromethylornithine), a specific inhibitor of polyamine synthesis, on intestinal regeneration. Forty-eight New Zealand white rabbits had 2 x 5-cm ileal defects patched with adjacent cecal serosal surface. One half of the animals took 2% eflorithine in drinking water postoperatively. Six animals in each group were killed 7, 14, 21, and 28 days after patching. There was no significant difference in neomucosal growth at any time. Villous height, disaccharidase activity, and crypt cell production were significantly lower in the eflorithine-treated animals. Eflorithine-treated animals had significantly lower ornithine decarboxylase activity and polyamine levels. Despite the inhibitory effect of eflorithine on polyamine synthesis and proliferative activity, epithelialization and contraction of the patched defect were not affected. These findings suggest that polyamine synthesis is important in proliferation and differentiation of cells in the neomucosa but does not influence cell migration in intestinal regeneration.
用相邻的浆膜表面修补肠道缺损会促使新的肠黏膜生长。由于多胺生物合成与细胞生长和分化相关，其在这一再生过程中可能很重要。我们的目的是确定多胺合成的特异性抑制剂依氟鸟氨酸（二氟甲基鸟氨酸）对肠道再生的影响。48只新西兰白兔的2×5厘米回肠缺损用相邻的盲肠浆膜表面进行修补。其中一半动物术后饮用含2%依氟鸟氨酸的水。每组6只动物在修补后7、14、21和28天处死。在任何时间点，新黏膜生长均无显著差异。依氟鸟氨酸处理组动物的绒毛高度、双糖酶活性和隐窝细胞生成均显著降低。依氟鸟氨酸处理组动物的鸟氨酸脱羧酶活性和多胺水平显著降低。尽管依氟鸟氨酸对多胺合成和增殖活性有抑制作用，但修补缺损处的上皮化和收缩并未受到影响。这些发现表明，多胺合成在新黏膜细胞的增殖和分化中很重要，但不影响肠道再生中的细胞迁移。
