Dutruel Céline, Thole Jelle, Geels Mark, Mollenkopf Hans-Joachim, Ottenhoff Tom, Guzman Carlos A, Fletcher Helen A, Leroy Odile, Kaufmann Stefan H E
European Vaccine Initiative, Heidelberg University Hospital, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany.
Tuberculosis Vaccine Initiative, Runderweg 6, 8219 PK Lelystad, The Netherlands.
Vaccine. 2014 Jul 31;32(35):4365-4368. doi: 10.1016/j.vaccine.2014.06.014. Epub 2014 Jun 17.
High-throughput analyses of RNA and protein expression are increasingly used for better understanding of vaccine-induced immunity and protection against infectious disease. With an increasing number of vaccine candidates in clinical development, it is timely to consider standardisation and harmonisation of sample collection, storage and analysis to ensure results of highest quality from these precious samples. These challenges were discussed by a group of international experts during a workshop organised by TRANSVAC, a European Commission-funded Research Infrastructure project. The main conclusions were: Platforms are rarely standardised for use in preclinical and clinical studies. Coordinated efforts should continue to harmonise the experimental set up of these studies, as well as the establishment of internal standards and controls. This will ensure comparability, efficiency and feasibility of the global analyses performed on preclinical and clinical data sets.
对RNA和蛋白质表达进行高通量分析越来越多地用于更好地理解疫苗诱导的免疫以及对传染病的防护。随着临床开发中候选疫苗数量的不断增加，现在是时候考虑样本采集、储存和分析的标准化与协调统一，以确保从这些珍贵样本中获得最高质量的结果。在由欧盟委员会资助的研究基础设施项目TRANSVAC组织的一次研讨会上，一群国际专家讨论了这些挑战。主要结论如下：在临床前和临床研究中，平台很少进行标准化使用。应继续做出协调努力，以统一这些研究的实验设置以及内部标准和对照的建立。这将确保对临床前和临床数据集进行的全球分析具有可比性、效率和可行性。
