Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
Bioresour Technol. 2014 Aug;166:541-7. doi: 10.1016/j.biortech.2014.05.097. Epub 2014 Jun 3.
The present study investigated the effects of several physicochemical parameters on the improvement of phycobiliproteins (especially phycocyanin) synthesis in a newly isolated species of Nostoc sp. Standard BG11₀ medium was modified to enhance the biomass productivity in different photobioreactors. The initial pH of 8, light intensity of 40 μmol m(-2)s(-1), temperature of 35 °C, diurnal cycle of 16:8 h (light:dark regime), 75.48 μM Na₂CO₃ and 17.65 mM NaNO₃ were found most suitable for the phycobiliproteins synthesis. Cyanobacteria exhibited chromatic adaptation, causing overexpression of phycocyanin in red and phycoerythrin in green light. The maximum phycobiliproteins yield of 0.13 gg(-1) dry cell weight was obtained in green light. Phycocyanin was further purified using thin layer chromatography (TLC), anion exchange chromatography and SDS-PAGE (denaturing gel) electrophoresis.
本研究考察了几种物理化学参数对新分离的念珠藻属（ Nostoc sp.）物种中藻蓝蛋白（尤其是藻蓝蛋白）合成的影响。对标准 BG11₀培养基进行了改良，以提高不同光生物反应器中的生物量生产率。初始 pH 值为 8、光强度为 40 μmol m(-2)s(-1)、温度为 35°C、昼夜周期为 16:8 h（光照：黑暗阶段）、75.48 μM Na₂CO₃ 和 17.65 mM NaNO₃ 最适合藻蓝蛋白的合成。蓝藻表现出色适应，导致藻蓝蛋白在红光和藻红蛋白在绿光中过度表达。在绿光下获得了最高的藻蓝蛋白产量为 0.13 gg(-1)干细胞重量。藻蓝蛋白进一步使用薄层色谱（TLC）、阴离子交换色谱和 SDS-PAGE（变性凝胶）电泳进行纯化。
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