Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699-5810, USA.
Adv Exp Med Biol. 2014;806:331-40. doi: 10.1007/978-3-319-06068-2_15.
The zebrafish (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafish producing mutants in a vast array of genes. While genetic manipulation of zebrafish has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a-/- and WT intestine.
斑马鱼（Danio rerio）经常被用于研究人类疾病的遗传学以及由此产生的病理学。由于其易于进行正向和反向遗传操作，并且保留了脊椎动物器官系统和致病基因，因此该系统成为了一种流行的模型。已经开发出许多技术来操纵斑马鱼的基因组，从而产生大量基因的突变体。尽管斑马鱼的遗传操作已经取得了进展，但蛋白质组学的应用却一直受到限制。本文综述了已经使用蛋白质组学技术进行的研究，以及我们最初使用蛋白质组学技术进行的比较 ascl1a-/-和 WT 肠之间蛋白质组变化的工作。
