蛋白质组学中的瓶颈。

Bottlenecks in proteomics.

机构信息

Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY, 13699-5810, USA.

出版信息

Adv Exp Med Biol. 2014;806:581-93. doi: 10.1007/978-3-319-06068-2_29.

DOI:10.1007/978-3-319-06068-2_29

PMID:24952204

Abstract

Mass spectrometry (MS) is the core for advanced methods in proteomic experiments. When effectively used, proteomics may provide extensive information about proteins and their post-translational modifications, as well as their interaction partners. However, there are also many problems that one can encounter during a proteomic experiment, including, but not limited to sample preparation, sample fractionation, sample analysis, data analysis & interpretation, and biological significance. Here we discuss some of the problems that researchers should be aware of when performing a proteomic experiment.

摘要

质谱（MS）是蛋白质组实验高级方法的核心。如果有效地使用，蛋白质组学可以提供关于蛋白质及其翻译后修饰以及其相互作用伙伴的广泛信息。然而，在蛋白质组实验中也会遇到许多问题，包括但不限于样品制备、样品分级、样品分析、数据分析和解释以及生物学意义。在这里，我们讨论了研究人员在进行蛋白质组实验时应该注意的一些问题。