Gerritsen M E, Rimarachin J, Perry C A, Weinstein B I
Department of Physiology, New York Medical College, Valhalla 10595.
Invest Ophthalmol Vis Sci. 1989 Apr;30(4):698-705.
Pathways of arachidonic acid metabolism were identified in freshly prepared and in cultured bovine corneal endothelial cells. The principal pathway of arachidonic acid metabolism in the bovine corneal endothelial cells appears to be the cyclooxygenase pathway with the resultant synthesis of PGI2, PGF2 alpha and PGE2. At least two of these products, PGI2 and PGF2 alpha, are formed by the enzymatic conversion of the substrate, PGH2. Measurements of endogenous prostaglandin production by radioimmunoassay demonstrated that PGE2 was the major arachidonic acid metabolite released, with smaller amounts of PGF2 alpha and the stable hydrolysis product of PGI2, 6-keto PGF1 alpha. The release of all three prostanoids was significantly increased by the addition of the calcium ionophore (A23187), human thrombin, bradykinin and histamine. Basal and stimulated release of prostaglandins by the corneal endothelium may contribute to the regulation of intraocular pressure and also in the modulation of the corneal response to injury.
在新鲜制备的和培养的牛角膜内皮细胞中确定了花生四烯酸代谢途径。牛角膜内皮细胞中花生四烯酸代谢的主要途径似乎是环氧化酶途径，最终合成前列环素（PGI2）、前列腺素F2α（PGF2α）和前列腺素E2（PGE2）。这些产物中至少有两种，即PGI2和PGF2α，是由底物前列腺素H2（PGH2）经酶促转化形成的。通过放射免疫测定法对内源性前列腺素产生的测量表明，PGE2是释放的主要花生四烯酸代谢产物，PGF2α和PGI2的稳定水解产物6-酮-前列腺素F1α（6-keto PGF1α）的量较少。添加钙离子载体（A23187）、人凝血酶、缓激肽和组胺后，所有三种前列腺素的释放均显著增加。角膜内皮细胞中前列腺素的基础释放和刺激释放可能有助于调节眼压，也有助于调节角膜对损伤的反应。
