College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350108, Fujian, China.
Biosens Bioelectron. 2014 Nov 15;61:575-8. doi: 10.1016/j.bios.2014.05.061. Epub 2014 Jun 9.
A new biosensing platform based on electrospun carbon nanotubes nanofibers (CNTs@PNFs) composite, which enabled strong electrochemiluminescent emission of peroxydisulfate, was firstly developed for immunoassay with favorable analytical performances, and then was utilized to evaluate the interaction between antibody and antigen in vitro. Moreover, the obvious ECL image of peroxydisulfate on the prepared sensing platform was firstly recorded in this report. In order to expand the application of peroxydisulfate ECL, the specific recognization biomolecules, α-fetoprotein (AFP) antibody was bound to the functionalized film via electrostatic interaction for fabricating label-free ECL immunosensor to detect α-AFP. Based on the ECL change resulting from the specific immunoreaction between antigen and antibody, the quantitative analysis for AFP with wide dynamic response in the range from 0.1 pg mL(-1) to 160 ng mL(-1) was realized. And the limit of detection was estimated to be 0.09 pg mL(-1). Therefore, the flexible sensing platform not only acted as the sensitized sensing element, but also offered a suitable carrier for immobilization of biological recognition elements with low-toxicity and eco-friendliness, which opened a promising approach to developing further electrospun nanofiber based amplified ECL biosensor with favorable analytical performances.
基于静电纺丝碳纳米管纳米纤维（CNTs@PNFs）复合材料的新型生物传感平台，可增强过二硫酸盐的电化学发光发射，首次被开发用于具有良好分析性能的免疫分析，然后用于体外评估抗体和抗原之间的相互作用。此外，本报告首次记录了制备的传感平台上过二硫酸盐的明显 ECL 图像。为了扩展过二硫酸盐 ECL 的应用，通过静电相互作用将特异性识别生物分子，即甲胎蛋白（AFP）抗体结合到功能化薄膜上，用于构建无标记 ECL 免疫传感器以检测 α-AFP。基于抗原和抗体之间特异性免疫反应引起的 ECL 变化，实现了对 AFP 的宽动态响应范围为 0.1 pg mL(-1) 至 160 ng mL(-1)的定量分析。检测限估计为 0.09 pg mL(-1)。因此，柔性传感平台不仅充当敏化传感元件，而且还为固定化生物识别元件提供了合适的载体，具有低毒性和生态友好性，为进一步开发具有良好分析性能的基于静电纺丝纳米纤维的放大 ECL 生物传感器开辟了有希望的途径。
