Aizen Joseph, Kowalsman Noga, Niv Masha Y, Levavi-Sivan Berta
The Robert H. Smith Faculty of Agriculture, Food and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Gen Comp Endocrinol. 2014 Oct 1;207:28-33. doi: 10.1016/j.ygcen.2014.05.028. Epub 2014 Jun 2.
In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.
在鱼类中，促卵泡激素（FSH）和促黄体生成素（LH）在生殖过程中都发挥着重要作用。在此，我们探究了罗非鱼（t）促性腺激素（GTHs）的结构和差异特异性，以阐明它们的生理相关性及其调节本质。我们构建了tGTHs和GTH受体（R）的结构模型，这使我们能够更好地理解激素 - 受体相互作用区域。在罗非鱼中，FSH的释放受下丘脑十肽GnRH的控制，这种作用可被特异性生物中和抗血清[抗重组（r）tFSHβ]消除。这些抗血清还降低了基础分泌，并延迟了GnRH刺激的11 - 酮睾酮（11KT）的产生，且显著降低了LH水平。使用抗rtLHβ对tLH进行免疫中和显著降低了其GnRH刺激的水平。基础11KT和FSH水平也有所降低。综上所述，这些结果表明罗非鱼中FSH和LH释放之间存在反馈机制。
