Mallepally Rajendar R, Marin Michael A, McHugh Mark A
Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
Acta Biomater. 2014 Oct;10(10):4419-24. doi: 10.1016/j.actbio.2014.06.007. Epub 2014 Jun 20.
Biocompatible and biodegradable porous materials based on silk fibroin (SF), a natural protein derived from the Bombyx mori silkworm, are being extensively investigated for use in biomedical applications including mammalian cell bioprocessing, tissue engineering and drug delivery applications. In this work, low-pressure, gaseous CO2 is used as an acidifying agent to fabricate SF hydrogels. This low-pressure CO2 acidification method is compared to an acidification method using high-pressure CO2 to demonstrate the effect of CO2 mass transfer and pressure on SF sol-gel kinetics. The effect of SF molecular weight on the sol-gel kinetics is determined using the low-pressure CO2 method. The results from these studies demonstrate that low-pressure CO2 processing proves to be a facile method for synthesizing 3-D SF hydrogels.
基于丝素蛋白（SF）的生物相容性和可生物降解的多孔材料正被广泛研究用于生物医学应用，包括哺乳动物细胞生物加工、组织工程和药物递送应用。丝素蛋白是一种从家蚕中提取的天然蛋白质。在这项工作中，低压气态二氧化碳被用作酸化剂来制备丝素蛋白水凝胶。将这种低压二氧化碳酸化方法与使用高压二氧化碳的酸化方法进行比较，以证明二氧化碳传质和压力对丝素蛋白溶胶-凝胶动力学的影响。使用低压二氧化碳方法确定丝素蛋白分子量对溶胶-凝胶动力学的影响。这些研究结果表明，低压二氧化碳处理被证明是合成三维丝素蛋白水凝胶的一种简便方法。
