Al-Rasheed Nouf M, Al-Rasheed Nawal M, Faddah L M, Mohamed Azza M, Mohammad Raeesa A, Al-Amin Maha
Pharmacology Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Biochemistry Department, Faculty of Science for Girls, King Abdulaziz University, P.O Box 51459, Jeddah 21453, Saudi Arabia ; Theraputic Chemistry Department, National Research Center, Dokki, Egypt.
Saudi J Biol Sci. 2014 Jul;21(3):265-74. doi: 10.1016/j.sjbs.2013.09.006. Epub 2013 Sep 17.
The aim of this study was to investigate the effective role of silymarin either alone or in combination with chlorogenic acid and/or melatonin against the toxic impact of carbon tetrachloride (CCl4) induced cardiac infarction. CCl4 (l.2 ml/kg body weight) was administered as a single dose intraperitoneally. The results revealed that the administration of silymarin alone or in combination with chlorogenic acid (CGA) and/or melatonin for 21 consecutive days, 24 h after CCl4 injection to rats, markedly ameliorated the increases in serum markers of cardiac infarction, including troponin T and creatine kinase-MB (CK-MB), as well as increases in the pro-inflammatory biomarkers, including interleukin-6 (IL-6), interferon-γ (IFN-γ) in serum and tumor necrosis factor-α (TNF-α) and C-reactive protein in cardiac tissue compared to CCl4 intoxicated rats. The used agents also successfully modulated the alteration in vascular endothelial growth factor (VEGF) in serum and the oxidative DNA damage and the increase in the apoptosis marker caspase 3 in cardiac tissue in response to CCl4 toxicity. The present biochemical results are supported by histo-pathological examination. The current results proved that treatment with silymarin in combination with CGA and melatonin was the most effective one in ameliorating the toxicity of CCl4 induced cardiac damage and this may support the use of this combination as an effective drug to treat cardiac damage induced by toxic agents.
本研究的目的是调查水飞蓟素单独使用或与绿原酸和/或褪黑素联合使用对四氯化碳（CCl4）诱导的心肌梗死毒性影响的有效作用。以1.2毫升/千克体重的剂量腹腔注射给予CCl4单剂量。结果显示，在给大鼠注射CCl4后24小时，连续21天单独给予水飞蓟素或与绿原酸（CGA）和/或褪黑素联合使用，可显著改善心肌梗死血清标志物的升高，包括肌钙蛋白T和肌酸激酶同工酶MB（CK-MB），以及促炎生物标志物的升高，包括血清中的白细胞介素-6（IL-6）、干扰素-γ（IFN-γ）和心脏组织中的肿瘤坏死因子-α（TNF-α）和C反应蛋白，与CCl4中毒大鼠相比。所用药物还成功调节了血清中血管内皮生长因子（VEGF）的变化以及心脏组织中氧化DNA损伤和凋亡标志物半胱天冬酶3的增加，以应对CCl4毒性。目前的生化结果得到了组织病理学检查的支持。目前的结果证明，水飞蓟素与CGA和褪黑素联合治疗在改善CCl4诱导的心脏损伤毒性方面是最有效的，这可能支持将这种联合用药作为治疗有毒物质诱导的心脏损伤的有效药物。
