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二肽基肽酶 I 通过加工表面活性蛋白 D 来控制肺炎克雷伯菌肺部感染的存活。

Dipeptidyl peptidase I controls survival from Klebsiella pneumoniae lung infection by processing surfactant protein D.

机构信息

Department of Medicine and The Cardiovascular Research Institute, University of California, San Francisco, CA 94143-0111.

出版信息

Biochem Biophys Res Commun. 2014 Jul 18;450(1):818-823. doi: 10.1016/j.bbrc.2014.06.062. Epub 2014 Jun 21.

DOI:10.1016/j.bbrc.2014.06.062
PMID:24955853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4107110/
Abstract

Prior work established that a deficiency in the cysteine protease dipeptidyl peptidase I (DPPI) improves survival following polymicrobial septic peritonitis. To test whether DPPI regulates survival from severe lung infections, DPPI(-/-) mice were studied in a Klebsiella pneumoniae lung infection model, finding that survival in DPPI(-/-) mice is significantly better than in DPPI(+/+) mice 8d after infection. DPPI(-/-) mice have significantly fewer bacteria in the lung than infected DPPI(+/+) mice, but no difference in lung histopathology, lung injury, or cytokine levels. To explore mechanisms of enhanced bacterial clearance in DPPI(-/-) mice, we examined the status of pulmonary collectins, finding that levels of surfactant protein D, but not of surfactant protein A, are higher in DPPI(-/-) than in DPPI(+/+) BAL fluid, and that DPPI(-/-) BAL fluid aggregate bacteria more effectively than control BAL fluid. Sequencing of the amino terminus of surfactant protein D revealed two or eight additional amino acids in surfactant protein D isolated from DPPI(-/-) mice, suggesting processing by DPPI. These results establish that DPPI is a major determinant of survival following Klebsiella pneumoniae lung infection and suggest that the survival disadvantage in DPPI(+/+) mice is in part due to processing of surfactant protein D by DPPI.

摘要

先前的工作表明，半胱氨酸蛋白酶二肽基肽酶 I（DPPI）的缺乏可改善多微生物脓毒症性腹膜炎后的存活率。为了测试 DPPI 是否调节严重肺部感染的存活率，在肺炎克雷伯菌肺部感染模型中研究了 DPPI（-/-）小鼠，发现 DPPI（-/-）小鼠的存活率明显优于感染后 8 天的 DPPI（+/+）小鼠。DPPI（-/-）小鼠肺部的细菌明显少于感染 DPPI（+/+）小鼠，但肺部组织病理学、肺损伤或细胞因子水平没有差异。为了探索 DPPI（-/-）小鼠中增强细菌清除的机制，我们检查了肺表面活性剂蛋白的状态，发现 DPPI（-/-）比 DPPI（+/+） BAL 液中的表面活性剂蛋白 D 水平更高，但表面活性剂蛋白 A 没有差异，并且 DPPI（-/-）BAL 液比对照 BAL 液更有效地聚集细菌。表面活性剂蛋白 D 的氨基末端测序显示，从 DPPI（-/-）小鼠中分离出的表面活性剂蛋白 D 有两个或八个额外的氨基酸，表明 DPPI 参与了其加工。这些结果表明 DPPI 是肺炎克雷伯菌肺部感染后存活的主要决定因素，并表明 DPPI（+/+）小鼠存活劣势部分是由于 DPPI 对表面活性剂蛋白 D 的加工。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/1d87ec3b2dd5/nihms-607522-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/71fa80a086fc/nihms-607522-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/0d4da42a17b8/nihms-607522-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/5e04f544fb16/nihms-607522-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/1d87ec3b2dd5/nihms-607522-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/71fa80a086fc/nihms-607522-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/0d4da42a17b8/nihms-607522-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/5e04f544fb16/nihms-607522-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57c1/4107110/1d87ec3b2dd5/nihms-607522-f0004.jpg

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J Immunol. 2008 Oct 15;181(8):5598-605. doi: 10.4049/jimmunol.181.8.5598.
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