Song Huayu, He Yan, Ma Liman, Zhou Xiaosu, Liu Xiumei, Qi Jie, Zhang Quanqi
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Gen Comp Endocrinol. 2015 Apr 1;214:114-25. doi: 10.1016/j.ygcen.2014.06.010. Epub 2014 Jun 20.
Kisspeptins are neuropeptides that play important roles in the reproduction and the onset of puberty in vertebrate by activating their receptor, Kissr. In the present study, we first isolated kiss1 and kissr4 genes from an ovoviviparous fish, the black rockfish (Sebastes schlegeli) by homologue cloning. Phylogenetic analysis indicated that the kiss and kissr of S. schlegeli belonged to kiss1 and kissr4 respectively. Quantitative real-time PCR analysis showed that the kissr4 was expressed mainly in the brain and testis, while the kiss1 was expressed predominantly in the heart of both sexes. As for the different gonadal maturation stages the kiss1 showed different expression patterns in different tissues. During the early development stage, expression levels of the ligand and receptor genes showed similar increasing trends. The promoter region of kissr4 contained several putative transcription factor (TF) binding sites which may have the function of regulating kisspeptin system gene expression, providing potential targets for future in-depth investigation. These results together confirmed that the kisspeptin system in S. schlegeli may be involved in reproduction and other activities. Furthermore, our study laid the groundwork for further learning about the evolution and function of kisspeptin system in fish even vertebrate.
亲吻素是一种神经肽，通过激活其受体Kissr在脊椎动物的生殖和青春期启动过程中发挥重要作用。在本研究中，我们首先通过同源克隆从卵胎生鱼类黑鲪（Sebastes schlegeli）中分离出kiss1和kissr4基因。系统发育分析表明，黑鲪的kiss和kissr分别属于kiss1和kissr4。定量实时PCR分析表明，kissr4主要在脑和睾丸中表达，而kiss1在两性的心脏中表达为主。至于不同性腺成熟阶段，kiss1在不同组织中表现出不同的表达模式。在早期发育阶段，配体和受体基因的表达水平呈现出相似的上升趋势。kissr4的启动子区域包含几个假定的转录因子（TF）结合位点，可能具有调节亲吻素系统基因表达的功能，为未来的深入研究提供了潜在靶点。这些结果共同证实，黑鲪中的亲吻素系统可能参与生殖和其他活动。此外，我们的研究为进一步了解鱼类甚至脊椎动物中亲吻素系统的进化和功能奠定了基础。
