Virological characterization of HIV-2 vpx gene mutants in various cell systems.

作者信息

Nomaguchi Masako, Doi Naoya, Adachi Akio

机构信息

Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.

Department of Microbiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan; Japanese Foundation for AIDS Prevention, Chiyoda-ku, Tokyo 101-0061, Japan.

出版信息

Microbes Infect. 2014 Aug;16(8):695-701. doi: 10.1016/j.micinf.2014.06.004. Epub 2014 Jun 21.

DOI:10.1016/j.micinf.2014.06.004

PMID:24956595

Abstract

Requirement of intrinsically disordered protein Vpx for HIV-2 replication is cell-type dependent. To define Vpx-dependent conditions, replication ability of HIV-2 vpx mutants was analyzed in various cell lines that differ in cellular type, differentiation state and/or expression level of anti-HIV-1 SAMHD1 degraded by Vpx. Induction of Vpx-sensitive anti-HIV-2 state was not always associated with SAMHD1 expression. Compared with our previous data in lymphocytic cells, growth-defectiveness of the vpx mutants in differentiated THP-1 cells, a newly established multi-cycle infection system, was considerably different. Taken together, our results suggest that Vpx plays cell-type dependent role through its undetermined structure and/or function.

摘要