Yang Peng-Fei, Liu Chao, Wang Hong-Qing, Li Jia-Chun, Wang Zhen-Zhong, Xiao Wei, Chen Ruo-Yun
Zhongguo Zhong Yao Za Zhi. 2014 Mar;39(6):1030-3.
The chemical constituents of Poria cocos were studied by means of silica gel, ODS column chromatography, Sephadex LH-20 and preparative HPLC. Thirteen compounds were isolated from this plant. By analysis of the ESI-MS and NMR data, the structures of these compounds were determined as tumulosic acid (1), dehydrotumulosic acid (2), 3beta, 5alpha-dihydroxy-ergosta-7, 22-dien-6-one (3), 3beta, 5alpha, 9alpha-trihydroxy-ergosta-7, 22-diene -6-one (4), ergosta-7, 22-diene-3-one (5), 6, 9-epoxy-ergosta-7,22-diene-3-ol (6), ergosta-4,22-diene-3-one (7), 3beta, 5alpha, 6beta-trihydroxyl-ergosta-7,22-diene (8), ergosta-5, 6-epoxy-7,22-dien-3-ol (9), beta-sitosterol (10), ribitol (11), mannitol (12), and oleanic acid 3-O-acetate (13), respectively. Compounds 3-13 were isolated from the P. cocos for the first time.
采用硅胶、ODS柱色谱、葡聚糖凝胶LH - 20和制备型高效液相色谱法对茯苓的化学成分进行了研究。从该植物中分离出13种化合物。通过对电喷雾电离质谱（ESI - MS）和核磁共振（NMR）数据的分析，确定这些化合物的结构分别为茯苓酸（1）、去氢茯苓酸（2）、3β,5α - 二羟基 - 麦角甾 - 7,22 - 二烯 - 6 - 酮（3）、3β,5α,9α - 三羟基 - 麦角甾 - 7,22 - 二烯 - 6 - 酮（4）、麦角甾 - 7,22 - 二烯 - 3 - 酮（5）、6,9 - 环氧 - 麦角甾 - 7,22 - 二烯 - 3 - 醇（6）、麦角甾 - 4,22 - 二烯 - 3 - 酮（7）、3β,5α,6β - 三羟基 - 麦角甾 - 7,22 - 二烯（8）、麦角甾 - 5,6 - 环氧 - 7,22 - 二烯 - 3 - 醇（9）、β - 谷甾醇（10）、核糖醇（11）、甘露醇（12）和齐墩果酸3 - O - 乙酸酯（13）。化合物3 - 13首次从茯苓中分离得到。
