Vonlanthen Mireille, Connelly Colleen M, Deiters Alexander, Linden Anthony, Finney Nathaniel S
Department of Chemistry, University of Zurich , Zurich CH-8057, Switzerland.
J Org Chem. 2014 Jul 3;79(13):6054-60. doi: 10.1021/jo500710g. Epub 2014 Jun 24.
We describe three significant advances in the use of thioureas as reporting elements for metal-responsive fluorescent chemosensors. First, on the basis of the crystal structure of a chemosensor analogue, we provide a deeper understanding of the details of the thiourea coordination environment. Second, we describe a new generation of chemosensors with higher affinities for Zn(2+) and Cd(2+) than were observed for earlier probes, expanding the scope of this type of probe beyond Hg(2+) detection. Third, we show that a thiourea-based chemosensor can be employed for fluorescence microscopy imaging of Hg(2+) ion concentrations in living mammalian cells.
我们描述了在使用硫脲作为金属响应荧光化学传感器的报告元素方面取得的三项重大进展。第一，基于化学传感器类似物的晶体结构，我们对硫脲配位环境的细节有了更深入的理解。第二，我们描述了新一代对Zn(2+)和Cd(2+)具有比早期探针更高亲和力的化学传感器，将这类探针的检测范围扩展到Hg(2+)之外。第三，我们表明基于硫脲的化学传感器可用于活的哺乳动物细胞中Hg(2+)离子浓度的荧光显微镜成像。
