Stieger Kai R, Feifel Sven C, Lokstein Heiko, Lisdat Fred
Biosystems Technology, Technical University of Applied Sciences Wildau, Hochschulring 1, D-15745 Wildau, Germany.
Phys Chem Chem Phys. 2014 Aug 7;16(29):15667-74. doi: 10.1039/c4cp00935e.
Conversion of light into an electrical current based on biohybrid systems mimicking natural photosynthesis is becoming increasingly popular. Photosystem I (PSI) is particularly useful in such photo-bioelectrochemical devices. Herein, we report on a novel biomimetic approach for an effective assembly of photosystem I with the electron transfer carrier cytochrome c (cyt c), deposited on a thiol-modified gold-surface. Atomic force microscopy and surface plasmon resonance measurements have been used for characterization of the assembly process. Photoelectrochemical experiments demonstrate a cyt c mediated generation of an enhanced unidirectional cathodic photocurrent. Here, cyt c can act as a template for the assembly of an oriented and dense layer of PSI and as wiring agent to direct the electrons from the electrode towards the photosynthetic reaction center of PSI. Furthermore, three-dimensional protein architectures have been formed via the layer-by-layer deposition technique resulting in a successive increase in photocurrent densities. An intermittent cyt c layer is essential for an efficient connection of PSI layers with the electrode and for an improvement of photocurrent densities.
基于模仿自然光合作用的生物杂交系统将光转化为电流正变得越来越流行。光系统I（PSI）在这种光生物电化学装置中特别有用。在此，我们报道了一种新颖的仿生方法，用于将光系统I与电子传递载体细胞色素c（cyt c）有效组装，该细胞色素c沉积在硫醇修饰的金表面上。原子力显微镜和表面等离子体共振测量已用于表征组装过程。光电化学实验证明了cyt c介导产生增强的单向阴极光电流。在此，cyt c可以作为组装定向且致密的PSI层的模板，并作为布线剂将电子从电极引向PSI的光合反应中心。此外，通过逐层沉积技术形成了三维蛋白质结构，导致光电流密度连续增加。间歇性的cyt c层对于PSI层与电极的有效连接以及光电流密度的提高至关重要。
