Wang Jing, Liu Yu-Tao, Xiao Lu, Zhu Lingpeng, Wang Qiujuan, Yan Tianhua
Department of Physiology and Pharmacology, China Pharmaceutical University, Tongjiaxiang 24, Nanjing, Jiangsu, 210009, China.
Inflammation. 2014 Dec;37(6):2085-90. doi: 10.1007/s10753-014-9942-x.
This study aims to evaluate the possible mechanisms responsible for the anti-inflammatory effects of apigenin lipopolysaccharide (LPS)-induced inflammatory in acute lung injury. In this study, the anti-inflammatory effects of apigenin on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and the possible mechanisms involved in this protection were investigated. Pretreatment with apigenin prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight/dry weight ratio in total leukocyte number and neutrophil percent in the bronchoalveolar lavage fluid (BALF) and in IL-6 and IL-1β, the tumor neurosis factor-α (TNF-α) in the BALF. These results showed that anti-inflammatory effects of apigenin against the LPS-induced ALI may be due to its ability of primary inhibition of cyclooxygenase-2 (COX-2) gene expression and nuclear factor kB (NF-kB) gene expression of lung. The results presented here suggest that the protective mechanism of apigenin may be attributed partly to decreased production of proinflammatory cytokines through the inhibition of COX-2 and NF-kB activation. The results support that use of apigenin is beneficial in the treatment of ALI.
本研究旨在评估芹菜素对脂多糖（LPS）诱导的急性肺损伤炎症产生抗炎作用的可能机制。在本研究中，研究了芹菜素对小鼠脂多糖（LPS）诱导的急性肺损伤（ALI）的抗炎作用以及这种保护作用涉及的可能机制。在气管内给予LPS之前用芹菜素预处理，可显著降低肺湿重/干重比、支气管肺泡灌洗液（BALF）中的总白细胞数和中性粒细胞百分比，以及BALF中的IL-6、IL-1β和肿瘤坏死因子-α（TNF-α）。这些结果表明，芹菜素对LPS诱导的ALI的抗炎作用可能归因于其对肺中环氧合酶-2（COX-2）基因表达和核因子κB（NF-κB）基因表达的初步抑制能力。此处呈现的结果表明，芹菜素的保护机制可能部分归因于通过抑制COX-2和NF-κB激活而减少促炎细胞因子的产生。这些结果支持芹菜素用于治疗ALI是有益的。
