Azoury R, Bitton M, Abrashkin S, Lavie E
Radiopharmaceuticals Department, Soreq Nuclear Research Center, Yavne, Israel.
Biochim Biophys Acta. 1989 May 1;995(3):295-300. doi: 10.1016/0167-4838(89)90050-2.
The kinetic behavior of fibrin clot lysis as induced by tissue-type plasminogen activator (t-PA) was studied using proton magnetic resonance (PMR) and a release assay of fibrin labeled with technetium-99m isotope (99mTc). The lysis pattern of the preformed clot was examined as a function of gradual changes in the amounts of added t-PA and deactivated t-PA. The behavior of fibrinolysis was found to depend strongly on the amount of t-PA in the assay, which markedly affects the lysis rate of fibrin. The changes induced by the lysis were reflected in pronounced prolongation of the transverse relaxation time. The PMR and the radioisotope release measurements point to the possibility that at least two steps are involved in the mechanism of lysis. The PMR seems to be associated with structural features of the clot and reflects the liberation of compartmentalized water from the clot, while the 99mTc analysis reflects the further fragmentation of fibrin.
利用质子磁共振（PMR）和用锝-99m同位素（99mTc）标记的纤维蛋白释放试验，研究了组织型纤溶酶原激活剂（t-PA）诱导的纤维蛋白凝块溶解的动力学行为。将预先形成的凝块的溶解模式作为添加的t-PA和失活t-PA量的逐渐变化的函数进行检查。发现纤维蛋白溶解行为强烈依赖于试验中t-PA的量，这显著影响纤维蛋白的溶解速率。溶解引起的变化反映在横向弛豫时间的明显延长上。PMR和放射性同位素释放测量结果表明，溶解机制中至少涉及两个步骤。PMR似乎与凝块的结构特征有关，并反映了凝块中分隔水的释放，而99mTc分析反映了纤维蛋白的进一步碎片化。
