Lapadula D M, Bowe M, Carrington C D, Dulak L, Friedman M, Abou-Donia M B
Department of Pharmacology, Duke University Medical Center, Durham, NC 27710.
Brain Res. 1989 Feb 27;481(1):157-61. doi: 10.1016/0006-8993(89)90496-4.
Acrylamide produces a dying back type of neuropathy in which there is an accumulation of neurofilaments in the axons. The in vitro binding of [14C]acrylamide to neurofilament and microtubule proteins obtained from rat spinal cord and brain was investigated. The relative binding to the high and middle molecular weight neurofilament was greater than to the low molecular weight neurofilament, while the rate of binding to MAP-1 (microtubule associated protein-1) and -2 was much greater than to tubulin. The binding rate to a 53 kDa protein which co-purified with the neurofilaments was between those of the middle and high molecular weight neurofilaments while the lowest rate of binding was to glial fibrillary acidic protein. These data indicate that there is a direct binding of acrylamide to cytoskeletal proteins.
丙烯酰胺会引发一种轴突逆行性神经病变，其特征是轴突中神经丝积聚。研究了[14C]丙烯酰胺与从大鼠脊髓和大脑中提取的神经丝及微管蛋白的体外结合情况。与高分子量和中分子量神经丝的相对结合量大于与低分子量神经丝的结合量，而与微管相关蛋白-1（MAP-1）和微管相关蛋白-2的结合速率远大于与微管蛋白的结合速率。与一种与神经丝共纯化的53 kDa蛋白的结合速率介于中分子量和高分子量神经丝之间，而与胶质纤维酸性蛋白的结合速率最低。这些数据表明丙烯酰胺与细胞骨架蛋白存在直接结合。
