Banik N L, Hogan E L, Whetstine L J, Balentine J D
Cent Nerv Syst Trauma. 1984 Winter;1(2):131-7. doi: 10.1089/cns.1984.1.131.
Calcium-induced myelopathy was produced in rats by dripping 1.0 ml of a 10% solution of CaCl2 at pH 7.4 upon exposed spinal cord. Changes in spinal cord proteins were examined following application of calcium. Analysis of proteins by SDS-PAGE revealed progressive losses of neurofilament, microtubular, and glial filament proteins over a period of 8 hours to 5 days. Large losses of myelin proteins were also evident. The protein alterations observed correlate well with ultrastructural changes and resemble those previously found with physical trauma. These observations indicate that Ca2+ plays a pivotal role, possibly by activating proteinase(s), in the degeneration of axons and myelin sheath in both Ca2+-induced myelopathy and spinal cord injury.
通过在暴露的脊髓上滴加1.0毫升pH值为7.4的10%氯化钙溶液，在大鼠中诱发钙诱导性脊髓病。在施加钙后检查脊髓蛋白质的变化。通过SDS-PAGE对蛋白质进行分析，结果显示在8小时至5天的时间内，神经丝、微管和神经胶质丝蛋白逐渐减少。髓磷脂蛋白也有大量损失。观察到的蛋白质变化与超微结构变化密切相关，并且类似于先前在物理创伤中发现的变化。这些观察结果表明，Ca2+可能通过激活蛋白酶，在钙诱导性脊髓病和脊髓损伤中轴突和髓鞘的退化过程中起关键作用。
