Nguyen T V, Juorio A V, Greenshaw A J
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
Brain Res Bull. 1989 Feb;22(2):197-200. doi: 10.1016/0361-9230(89)90043-9.
Striatal amine levels were measured six weeks after unilateral injections of 6-OHDA (8 micrograms) into the substantia nigra in male Wistar rats pretreated with monoamine oxidase inhibitors. After (-) deprenyl.HCl pretreatment (2 mg.kg-1 SC 2hr), beta-phenylethylamine, m- and p-tyramine ipsilateral to the 6-OHDA lesion decreased to 50, 18 and 25% of contralateral levels. DA, DOPAC and HVA also decreased on the lesioned side. Ipsilateral concentrations of tryptamine, 5-HT, 5-HIAA, p-tyrosine and L-tryptophan concentrations were equivalent to contralateral values in this condition. In animals pretreated with pargyline.HCl (200 mg.kg-1 IP 2hr) m- and p-tyramine and tryptamine ipsilateral to the lesion decreased to 48, 59 and 57% of contralateral levels. Ipsilateral DA decreased to 26% of the contralateral value. Under these conditions no change in concentrations of beta-phenylethylamine or of the above acid metabolites or amino acids was observed. The masking of lesion-induced changes in beta-phenylethylamine by pargyline is attributed to the lipophilic nature of of this molecule and consequent diffusion of this amine from other areas after maximal monoamine oxidase inhibition. Conversely the failure to demonstrate lesion-induced changes in tryptamine with (-) deprenyl pretreatment is attributed to the nonselectivity of monoamine oxidase for tryptamine and activity of monoamine oxidase A under these conditions. These results indicate that for further assessment of lesion-induced changes in beta-phenylethylamie and of tryptamine the respective (-) deprenyl and pargyline pretreatments used in this study are appropriate.(ABSTRACT TRUNCATED AT 250 WORDS)
在给用单胺氧化酶抑制剂预处理的雄性Wistar大鼠单侧黑质注射6 - 羟基多巴胺（8微克）六周后，测量纹状体胺水平。在（ - ）盐酸司来吉兰预处理（2毫克·千克⁻¹，皮下注射，2小时）后，6 - 羟基多巴胺损伤同侧的β-苯乙胺、间酪氨酸和对酪氨酸降至对侧水平的50%、18%和25%。损伤侧的多巴胺、二羟基苯乙酸和高香草酸也降低。在此条件下，损伤同侧的色胺、5 - 羟色胺、5 - 羟吲哚乙酸、对酪氨酸和L - 色氨酸浓度与对侧值相当。在用盐酸帕吉林（200毫克·千克⁻¹，腹腔注射，2小时）预处理的动物中，损伤同侧的间酪氨酸、对酪氨酸和色胺降至对侧水平的48%、59%和57%。损伤同侧的多巴胺降至对侧值的26%。在这些条件下，未观察到β-苯乙胺或上述酸性代谢产物及氨基酸浓度的变化。盐酸帕吉林对损伤诱导的β-苯乙胺变化的掩盖作用归因于该分子的亲脂性以及在单胺氧化酶最大抑制后该胺从其他区域的扩散。相反，（ - ）盐酸司来吉兰预处理未能显示损伤诱导的色胺变化归因于单胺氧化酶对色胺的非选择性以及在此条件下单胺氧化酶A的活性。这些结果表明，对于进一步评估损伤诱导的β-苯乙胺和色胺变化，本研究中使用的各自的（ - ）盐酸司来吉兰和盐酸帕吉林预处理是合适的。（摘要截短于250字）
