使用抗猪肝脏尿酸酶的哺乳动物尿酸酶比较免疫学

Comparative immunology of mammalian uricase using anti-hog liver uricase.

Htay H H, Sato K, Tamaki K, Tsutsumi H, Katsumata Y

Department of Legal Medicine, Nagoya University School of Medicine, Japan.

Comp Biochem Physiol B. 1989;92(3):583-6. doi: 10.1016/0305-0491(89)90135-1.

Abstract

Uricase was partially purified from livers of five mammals (dog, bovine, horse, house musk shrew and guinea pig), and its immunological properties were studied using anti-hog liver uricase prepared in rabbits. 2. Results obtained by immunodiffusion suggest that hog uricase has four sites of partial antigens (a, b, c, d), while the enzyme of other mammals has only part of them, and a cladogram of these six mammals is presented showing development of partial antigens. 3. Results obtained by immunoelectrophoresis suggest that the protein structure of house musk shrew uricase is considerably different from the other five mammals tested.

摘要

从五种哺乳动物（狗、牛、马、家麝鼩和豚鼠）的肝脏中部分纯化了尿酸酶，并使用兔制备的抗猪肝尿酸酶研究了其免疫学特性。2. 免疫扩散获得的结果表明，猪尿酸酶有四个部分抗原位点（a、b、c、d），而其他哺乳动物的酶只有其中一部分，并且给出了这六种哺乳动物的系统发育树，显示了部分抗原的发展。3. 免疫电泳获得的结果表明，家麝鼩尿酸酶的蛋白质结构与其他五种测试的哺乳动物有很大不同。