大肠杆菌70S核糖体和70S起始复合物中23S rRNA-蛋白质邻域的差异。含双功能Pt(II)试剂的探测。

Differences in 23 S rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent.

作者信息

Chistyakov P G, Venjaminova A G, Vladimirov S N, Graifer D M, Kazakov S A, Karpova G G

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division USSR Academy of Sciences.

出版信息

FEBS Lett. 1989 Mar 27;246(1-2):197-201. doi: 10.1016/0014-5793(89)80282-0.

DOI:10.1016/0014-5793(89)80282-0

PMID:2495985

Abstract

rRNA-protein cross-links in free E. coli 35S-labeled 70 S ribosomes and in the initiation complex 35S-labeled 70 S ribosome.AUGU6.fMet-tRNA(fMet) were studied with the aid of a new type of binuclear Pt(II) compound - dichlorotetra-ammine(1,6-hexamethylenediaminediplatinum++ +) dichloride. The use of this reagent allowed us to reveal differences in the rRNA-protein neighbourhood in free 70 S ribosomes and in the initiation complex. Proteins L3, L6, L23 and L25 were shown to cross-link to 23 S rRNA only in the initiation complex, whereas proteins L1, L13, L14, L16, L17, L18, L22, L28 and S1 did so in both free ribosomes and the complex. 16 S rRNA was found to be cross-linked preferentially to a single protein, S1, in both states of the ribosomes.

摘要

利用一种新型双核铂（II）化合物——二氯四氨（1,6 - 己二胺二铂++ +）二氯化物，对游离的大肠杆菌35S标记的70S核糖体以及起始复合物35S标记的70S核糖体.AUGU6.fMet - tRNA（fMet）中的rRNA - 蛋白质交联进行了研究。使用该试剂使我们能够揭示游离70S核糖体和起始复合物中rRNA - 蛋白质邻域的差异。结果表明，蛋白质L3、L6、L23和L25仅在起始复合物中与23S rRNA交联，而蛋白质L1、L13、L14、L16、L17、L18、L22、L28和S1在游离核糖体和复合物中均如此。在核糖体的两种状态下，均发现16S rRNA优先与单一蛋白质S1交联。