武冈铜灰鹅线粒体全基因组序列的测定与分析
Determination and analysis of the complete mitochondrial genome sequence of Wugangtong grey goose.
作者信息
Lin Qian, Jiang Gui-Tao, Cao Rong, Yun Long, Li Guo-Jun, Dai Qiu-Zhong, Zhang Shi-Rui, Hou De-Xing, He Xi
机构信息
a College of Animal Science and Technology, Hunan Agricultural University , Changsha , P.R. China and.
b Department of Animal Nutrition and Feeding Technology , Hunan Institute of Animal Science and Veterinary Medicine , Changsha , P.R. China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1008-9. doi: 10.3109/19401736.2014.926527. Epub 2014 Jun 24.
Mitochondrial DNA plays an important role in living organisms. In this study, the complete mitochondrial genome sequence of the Wugangtong grey goose was reported in Human Province first, which was determined through PCR-based method. The total length of the mitogenome is 16,740 bp. It contains the typical structure, including 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop region) as that of most other vertebrates. The overall composition of the mitogenome was estimated to be 30.22% for A, 22.67% for T, 32.05% for C and 15.07% for G. All the protein initiation codons are ATG, except for COX1, COX2 and ND5 are GTG, ND6 is CTA. The complete mitochondrial genome sequence of the Wugangtong grey goose will provides an important data set for the study in genetic mechanism of goose in Hunan province.
线粒体DNA在生物体内发挥着重要作用。在本研究中，首次报道了湖南省武冈铜鹅的完整线粒体基因组序列，该序列通过基于PCR的方法测定。线粒体基因组全长16740bp。它具有典型的结构，包括2个核糖体RNA基因、22个转运RNA基因、13个蛋白质编码基因和1个非编码控制区（D环区），与大多数其他脊椎动物相同。线粒体基因组的总体组成估计为：A占30.22%，T占22.67%，C占32.05%，G占15.07%。所有蛋白质起始密码子均为ATG，但COX1、COX2和ND5的起始密码子为GTG，ND6的起始密码子为CTA。武冈铜鹅的完整线粒体基因组序列将为湖南省鹅遗传机制的研究提供重要数据集。
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