Cox J M, Mac Rae I C
Department of Microbiology, University of Queensland, St Lucia, Australia.
J Appl Bacteriol. 1989 Feb;66(2):137-52. doi: 10.1111/j.1365-2672.1989.tb02463.x.
Lipolytic and proteolytic psychrotrophs were isolated from raw and pasteurized goats' milk, which had been stored at 5 degrees C for 7 d. The 241 strains isolated and 20 reference strains were examined by 149 biochemical, physiological, and morphological tests. The results yielded 195 characters suitable for taxonomic analysis. Computer-assisted complete linkage analysis, using the Jaccard coefficient, produced 22 phenons at 75% S. The results showed that Pseudomonas fluorescens was the predominant psychrotrophic bacterium, but that Pseudomonas fragi was dominant in some milk samples. Strains of Serratia liquefaciens and Flavobacterium balustinum were also identified.
从冷藏于5摄氏度达7天的生羊奶和巴氏杀菌羊奶中分离出了脂肪分解和蛋白水解嗜冷菌。对分离得到的241株菌株以及20株参考菌株进行了149项生化、生理和形态学测试。结果得到了195个适合进行分类分析的特征。利用杰卡德系数进行计算机辅助完全连锁分析,在相似度为75%时产生了22个表型群。结果表明,荧光假单胞菌是主要的嗜冷菌,但在一些牛奶样本中,易碎假单胞菌占主导地位。还鉴定出了液化沙雷氏菌和巴鲁斯塔黄杆菌的菌株。