Seale J, Miles C, Bodwell C E
Energy and Protein Nutrition Laboratory, Beltsville Human Nutrition Research Center, Maryland 20705.
J Appl Physiol (1985). 1989 Feb;66(2):644-52. doi: 10.1152/jappl.1989.66.2.644.
Attempts to estimate human energy expenditure by use of doubly labeled water have produced three methods currently used for calculating carbon dioxide production from isotope disappearance data: 1) the two-point method, 2) the regression method, and 3) the integration method. An ideal data set was used to determine the error produced in the calculated energy expenditure for each method when specific variables were perturbed. The analysis indicates that some of the calculation methods are more susceptible to perturbations in certain variables than others. Results from an experiment on one adult human subject are used to illustrate the potential for error in actual data. Samples of second void urine, 24-h urine, and breath collected every other day for 21 days are used to calculate the average daily energy expenditure by three calculation methods. The difference between calculated energy expenditure and metabolizable energy on a weight-maintenance diet is used to estimate the error associated with the doubly labeled water method.
1）两点法，2）回归法，3）积分法。使用理想数据集来确定当特定变量受到干扰时每种方法在计算能量消耗中产生的误差。分析表明，某些计算方法比其他方法更容易受到特定变量干扰的影响。对一名成年人类受试者进行的实验结果用于说明实际数据中存在误差的可能性。每隔一天收集21天的第二次排尿尿液、24小时尿液和呼出气体样本，通过三种计算方法来计算平均每日能量消耗。在维持体重饮食下计算的能量消耗与可代谢能量之间的差异用于估计与双标记水法相关的误差。
