Marin M G, Zwierzynski D, McBride R K, Culp D J
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York 14642.
J Appl Physiol (1985). 1989 Mar;66(3):1390-6. doi: 10.1152/jappl.1989.66.3.1390.
The purpose of this study was to examine the role of acetylcholinesterase on mucociliary transport by use of a potent anticholinesterase agent, soman, and potential antagonists, atropine (muscarinic antagonist) and pralidoxime (acetylcholinesterase reactivator). Initial measurements of mucociliary transport rate were obtained in anesthetized ferrets at 30-min intervals for 5.5 h. These rates remained constant at a mean of 18.2 +/- 1.0 (SE) mm/min. We studied the effects of intravenously administered soman (1-8 micrograms/kg) and observed a dose-related change in the rate of mucociliary transport [-1.1 +/- 2.7 (SE) mm/min after 1 microgram/kg, 9.8 +/- 2.9 mm/min after 5 micrograms/kg, and 14.4 +/- 4.3 mm/min after 8 micrograms/kg of soman]. Pretreatment with atropine completely prevented the response to soman, whereas pretreatment with pralidoxime did not significantly alter the response. We postulate that soman's effect on mucociliary transport relates directly to its cholinergic activity. Failure of pralidoxime to inhibit the effects of soman may relate to pralidoxime's inability to reactivate acetylcholinesterase successfully.
本研究的目的是通过使用一种强效抗胆碱酯酶剂梭曼以及潜在拮抗剂阿托品（毒蕈碱拮抗剂）和氯解磷定（乙酰胆碱酯酶复活剂）来研究乙酰胆碱酯酶在黏液纤毛运输中的作用。在麻醉的雪貂中每隔30分钟测量一次黏液纤毛运输速率，持续5.5小时。这些速率保持恒定，平均为18.2±1.0（标准误）毫米/分钟。我们研究了静脉注射梭曼（1 - 8微克/千克）的效果，并观察到黏液纤毛运输速率呈剂量相关变化[梭曼1微克/千克后为-1.1±2.7（标准误）毫米/分钟，5微克/千克后为9.8±2.9毫米/分钟，8微克/千克后为14.4±4.3毫米/分钟]。用阿托品预处理可完全阻止对梭曼的反应，而用氯解磷定预处理并未显著改变该反应。我们推测梭曼对黏液纤毛运输的影响与其胆碱能活性直接相关。氯解磷定未能抑制梭曼的作用可能与其无法成功复活乙酰胆碱酯酶有关。
